Publication:
Porphyromonas gingivalis elevated high-mobility group box 1 levels after myocardial infarction in mice

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

13492365

DOI

10.1536/ihj.16-500

Other identifier(s)

2-s2.0-85032175240

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

International Heart Journal. Vol 58, No.5 (2017), p.762-768

Suggested Citation

Srisuwantha R., Shiheido Y., Aoyama N., Sato H., Kure K., Laosrisin N., Izumi Y., Suzuki J.-I. Porphyromonas gingivalis elevated high-mobility group box 1 levels after myocardial infarction in mice. International Heart Journal. Vol 58, No.5 (2017), p.762-768. doi:10.1536/ihj.16-500 Retrieved from: https://hdl.handle.net/20.500.14740/4092

Title

Porphyromonas gingivalis elevated high-mobility group box 1 levels after myocardial infarction in mice

Author(s)

Srisuwantha R.
 Shiheido Y.
 Aoyama N.
 Sato H.
 Kure K.
 Laosrisin N.
 Izumi Y.
 Suzuki J.-I.

Abstract

High mobility group box 1 (HMGB1) is a nuclear protein released from necrotic cells, inducing inflammatory responses. Epidemiological studies suggested a possible association between periodontitis and cardiovascular diseases (CVDs). Due to tissue damage and necrosis of cardiac cells following myocardial infarction (MI), HMGB1 is released, activating an inflammatory reaction. However, it remains unclear whether periodontitis is also involved in myocardial damage. The purpose of this study was to determine the effect of the periodontal pathogen Porphyromonas gingivalis (P.g.) after MI in mice. C57BL/6J wild type mice in post-MI were inoculated with P.g. in the infected group (P.g.-inoculated MI group) and with phosphate buffer saline (PBS) in the control group (PBS-injected MI group). Plasma samples and twelve tissue samples from mice hearts after MI were obtained. We determined the expression of HMGB1 by ELISA and immunohistochemistry. The level of HMGB1 protein in the P.g.-inoculated MI group was significantly higher than in the PBS-injected MI group on day 5, but not on day 14. Immunohistochemistry analysis revealed that HMGB1 was mainly expressed in cardiomyocytes, immune cells, and vascular endothelial cells in the PBS-injected MI group, while HMGB1 was seen broadly in degenerated cardiomyocytes, extracellular fields, immune cells, and vascular endothelial cells in the P.g.-inoculated MI group. A significant increase in the number of HMGB1 positive cells was observed in the P.g.-inoculated MI group compared to the PBS-injected MI group. Infection with P.g. after MI enhanced myocardial HMGB1 expression. There is a possible relationship between periodontitis and post-infarction myocardial inflammation through HMGB-1. © 2017, International Heart Journal Association. All rights reserved.

Subject(s)

High mobility group B1 protein
 Biological marker
 High mobility group B1 protein
 HMGB1 protein, mouse
 Animal cell
 Animal model
 Animal tissue
 Article
 Bacterial growth
 Bacterial infection
 Controlled study
 Enzyme linked immunosorbent assay
 Heart infarction
 Histopathology
 Immunohistochemistry
 Male
 Mouse
 Nonhuman
 Periodontal disease
 Porphyromonas gingivalis
 Priority journal
 Protein expression
 Animal
 Bacteroidaceae infection
 Biosynthesis
 C57BL mouse
 Cardiac muscle
 Cardiac muscle cell
 Complication
 Disease model
 Isolation and purification
 Metabolism
 Microbiology
 Myocardial Infarction
 Pathology
 Porphyromonas gingivalis
 Animals
 Bacteroidaceae Infections
 Biomarkers
 Disease Models, Animal
 Enzyme-Linked Immunosorbent Assay
 HMGB1 Protein
 Immunohistochemistry
 Male
 Mice
 Mice, Inbred C57BL
 Myocardial Infarction
 Myocardium
 Myocytes, Cardiac
 Porphyromonas gingivalis

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URI

https://hdl.handle.net/20.500.14740/4092

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Scopus: Year 1983-2021

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