Publication:
Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

10504648

DOI

10.1016/j.fsi.2017.04.005

Other identifier(s)

2-s2.0-85017449346

Rights Holder(s)

Scopus

Bibliographic Citation

Fish and Shellfish Immunology. Vol 65, (2017), p.88-95

Suggested Citation

Boonrawd S., Mani R., Ponprateep S., Supungul P., Masrinoul P., Tassanakajon A., Rimphanitchayakit V. Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon. Fish and Shellfish Immunology. Vol 65, (2017), p.88-95. doi:10.1016/j.fsi.2017.04.005 Retrieved from: https://hdl.handle.net/20.500.14740/4153

Title

Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon

Author(s)

Boonrawd S.
 Mani R.
 Ponprateep S.
 Supungul P.
 Masrinoul P.
 Tassanakajon A.
 Rimphanitchayakit V.

Abstract

Spätzle is a signaling ligand in innate immune response that signals pathogenic infection via Toll receptor and Toll pathway into the cells for the synthesis of antimicrobial proteins. Herein, three PmSpӓtzle isoforms were identified in Penaeus monodon, namely PmSpz1, 2 and 3. The PmSpz1 was chosen for detailed study. The PmSpz1 gene was expressed in all nine tissues tested including the hemocytes, stomach, hepatopancreas, gill, lymphoid tissue, eyestalk, muscle, intestine and heart. Its expression was up-regulated upon white spot syndrome virus (WSSV) infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as a cleaved active form awaiting to activate the Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV infection. Injection of a recombinant active form of PmSpz1 into a normal shrimp activated the synthesis of crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5 indicating that the expression of all antimicrobial proteins but not penaeidin5 was under the regulation of Toll pathway. © 2017 Elsevier Ltd

Subject(s)

ALFPm3 protein
 Complementary DNA
 CrustinPm1 protein
 CrustinPm7 protein
 DNA fragment
 Isoprotein
 Penaeidin3 protein
 Penaeidin5 protein
 Pmspatzle 1
 Protein
 Toll like receptor
 Unclassified drug
 Antimicrobial cationic peptide
 Arthropod protein
 Complementary DNA
 Isoprotein
 Amino acid sequence
 Animal cell
 Animal tissue
 Article
 Blood cell
 Comparative study
 Controlled study
 Eye tissue
 Fenneropenaeus chinensis
 Gene expression
 Heart tissue
 Hemolymph
 Hepatopancreas
 Ichthyophthiriasis
 Intestine tissue
 Lymphoid tissue
 Muscle tissue
 Nonhuman
 Nucleotide sequence
 Penaeus monodon
 Protein analysis
 Signal transduction
 Stomach tissue
 Upregulation
 Western blotting
 White spot syndrome virus
 Animal
 Chemistry
 Gene expression
 Genetics
 Immunology
 Innate immunity
 Metabolism
 Molecular cloning
 Penaeidae
 Physiology
 Sequence alignment
 Virology
 Amino Acid Sequence
 Animals
 Antimicrobial Cationic Peptides
 Arthropod Proteins
 Base Sequence
 Cloning, Molecular
 DNA, Complementary
 Gene Expression
 Hemocytes
 Immunity, Innate
 Penaeidae
 Protein Isoforms
 Sequence Alignment
 White spot syndrome virus 1

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URI

https://hdl.handle.net/20.500.14740/4153

Collections

Scopus: Year 1983-2021

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