Publication:
Cytokine responses during exacerbation compared with stable phase in asthmatic children

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

0125877X

Other identifier(s)

2-s2.0-77954547977

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Asian Pacific Journal of Allergy and Immunology. Vol 28, No.1 (2010), p.35-40

Suggested Citation

Charoenying Y., Kamchaisatian W., Atamasirikul K., Direkwattanachai C., Manuyakorn W., Benjaponpitak S. Cytokine responses during exacerbation compared with stable phase in asthmatic children. Asian Pacific Journal of Allergy and Immunology. Vol 28, No.1 (2010), p.35-40. Retrieved from: https://hdl.handle.net/20.500.14740/7611

Title

Cytokine responses during exacerbation compared with stable phase in asthmatic children

Author(s)

Charoenying Y.
 Kamchaisatian W.
 Atamasirikul K.
 Direkwattanachai C.
 Manuyakorn W.
 Benjaponpitak S.

Abstract

Bronchial asthma is a chronic inflammatory disorder of the airways. Balancing in Th1 and Th2 response is a target in the treatment. Recent studies show that interleukin-10 (IL-10) has an important role in the regulation of Th2 and allergic responses and its amount was found to decrease in asthmatic patients. This study was to focus on cytokine responses, including interferon-gamma (IFN- γ), IL-4 and IL-10 in asthmatic children during acute exacerbation compared to stable period. Peripheral blood mononuclear cells (PBMCs) from fourteen asthmatic children during exacerbation and stable phase were stimulated with phytohemagglutinin (PHA) and mite allergen (Der p) for 72 hours. Levels of IFN-γ, IL-4 and IL-10 in cell culture supernatants were measured using enzyme-linked immunosorbent assay. The median level of IL-10 in PBMCs stimulated with PHA was significantly lower in acute asthma exacerbation compared with stable phase (464 vs. 859.5 pg/ml, p= 0.03). However, there was no difference in the level of IL-10 in PBMCs stimulated with Der p. The level of IFN-γ and IL-4 were not different between exacerbation and stable phase both in PHA and Der p-stimulated PBMCs. The decrease of IL-10 pro-duction in asthmatic children during acute exacerbation may emphasize the role of IL-10 in immune regulation in allergic disease.

Subject(s)

Corticosteroid
 Gamma interferon
 House dust allergen
 Interleukin 10
 Interleukin 4
 Phytohemagglutinin
 Adolescent
 Article
 Asthma
 Cell level
 Cell stimulation
 Child
 Clinical article
 Controlled study
 Cytokine production
 Cytokine response
 Disease exacerbation
 Enzyme linked immunosorbent assay
 Female
 Human
 Human cell
 Immunoregulation
 Male
 Peripheral blood mononuclear cell
 School child
 Supernatant
 Adolescent
 Antigens, Dermatophagoides
 Asthma
 Cells, Cultured
 Child
 Cytokines
 Disease Progression
 Female
 Humans
 Leukocytes, Mononuclear
 Male
 Phytohemagglutinins
 Severity of Illness Index
 Skin Tests

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URI

https://hdl.handle.net/20.500.14740/7611

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Scopus: Year 1983-2021

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