Publication:
Influence of capsicum extract and capsaicin on endothelial health

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-79952271566

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 93, No.SUPPL 2 (2010), p.S92-S101

Suggested Citation

Chularojmontri L., Suwatronnakorn M., Wattanapitayakul S.K. Influence of capsicum extract and capsaicin on endothelial health. Journal of the Medical Association of Thailand. Vol 93, No.SUPPL 2 (2010), p.S92-S101. Retrieved from: https://hdl.handle.net/20.500.14740/7477

Title

Influence of capsicum extract and capsaicin on endothelial health

Author(s)

Chularojmontri L.
 Suwatronnakorn M.
 Wattanapitayakul S.K.

Abstract

Objective: To examine the effect of Capsicum spp extract (CEX) and capsaicin (CAP) on endothelial nitric oxide release and protection against lipopolysaccharide (LPS)-induced cellular apoptosis. Material and Method: Human umbilical vein endothelial cells (HUVEC) were isolated from newborn cords. Evaluation of cytotoxicity was performed by MTT assay. Endothelial nitric oxide (NO) production was evaluated by Griess reaction. Alteration in eNOS expression was detected by westernblot analysis. To induce oxidative stress and apoptosis, lipopolysaccharide (LPS) was coincubated with HUVEC in the presence or absence of CEX or CAP, and the vanilloid receptor blocker capsazepine (CZP). Hoechst nuclear staining was used to determine percent apoptotic nuclei. Results: The highest concentrations of CEX (1000 μg/mL) and CAP (25 μM) used in the study did not induce cytotoxicity in HUVEC. Significant increase in NO release was observed when cells were incubated with CEX (100 μg/mL) and CAP (25 μM) and this effect was inhibited by CZP only in CAP treatment group. Despite enhanced NO generation was observed, western blot analysis indicated no change in eNOS expression. Interestingly, endothelial cells incubated with L-arginine (LARG, 1000 μg/mL) alone significantly showed increased NO production while L-ARG co-incubation abrogated CEX or CAP effects on endothelial NO generation. CEX (10 μg/mL) and CAP (1μM) decreased apoptotic nuclei in HUVEC treated with LPS. Conclusion: CEX and CAP improved endothelial function and protected against LPS-induced apoptosis. Regular consumption of Capsicum spp. may promote endothelial health and reduce cardiovascular disease risk.

Subject(s)

Capsaicin
 Endothelial nitric oxide synthase
 Lipopolysaccharide
 Nitric oxide
 NOS3 protein, human
 Plant extract
 Apoptosis
 Article
 Biosynthesis
 Cell culture
 Chemistry
 Cytology
 Dose response
 Drug effect
 Endothelium cell
 Health
 Human
 Male
 Metabolism
 Newborn
 Oxidative stress
 Pepper
 Umbilical cord
 Umbilical vein
 Western blotting
 Apoptosis
 Blotting, Western
 Capsaicin
 Capsicum
 Cells, Cultured
 Dose-Response Relationship, Drug
 Endothelial Cells
 Health
 Humans
 Infant, Newborn
 Lipopolysaccharides
 Male
 Nitric Oxide
 Nitric Oxide Synthase Type III
 Oxidative Stress
 Plant Extracts
 Umbilical Cord
 Umbilical Veins

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URI

https://hdl.handle.net/20.500.14740/7477

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Scopus: Year 1983-2021

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