Publication:
Protective effect of purple corn silk extract against ultraviolet-B-induced cell damage in human keratinocyte cells

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

1105558

DOI

10.4103/japtr.JAPTR_238_20

Other identifier(s)

2-s2.0-85105407118

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Advanced Pharmaceutical Technology and Research. Vol 12, No.2 (2021), p.140-146

Suggested Citation

Poorahong W., Innalak S., Ungsurungsie M., Watanapokasin R. Protective effect of purple corn silk extract against ultraviolet-B-induced cell damage in human keratinocyte cells. Journal of Advanced Pharmaceutical Technology and Research. Vol 12, No.2 (2021), p.140-146. doi:10.4103/japtr.JAPTR_238_20 Retrieved from: https://hdl.handle.net/20.500.14740/7837

Title

Protective effect of purple corn silk extract against ultraviolet-B-induced cell damage in human keratinocyte cells

Author(s)

Poorahong W.
 Innalak S.
 Ungsurungsie M.
 Watanapokasin R.

Abstract

Ultraviolet-B (UVB) could lead to inflammation and cell death induction. Purple corn silk (PCS), part of female flower of corn has multiple pharmacological properties. This investigation focused on determining the preventive effects of PCS extract on human keratinocyte HaCaT cell damage induced by UVB irradiation. Cells were irradiated with 25 mJ/cm2UVB after pre-treated with PCS extract for 1 h. Then, the cells were then placed in culture medium followed by subsequent experiments. Cell survival was determined by MTT assay. The immunofluorescence, DCFH-DA, JC-1, and Hoeshst33342 staining assays were used to determine γ-H2AX, intracellular reactive oxygen species (ROS), membrane potential of mitochondria, and nuclear condensation, respectively. Western blot analysis was used to investigate the proteins expression. The statistically significant comparison was calculated by analysis of variance at P < 0.05. The fluorescence and protein band intensity were quantified by Image J densitometer. The results indicated cell survival was increased upon PCS extract pretreatment followed by UVB exposure. PCS extract decreased γ-H2AX expression, intracellular ROS overproduction, and nuclear condensation in cells induced by UVB. Furthermore, The PCS extract pretreatment attenuated apoptosis response through stabilized mitochondrial membrane potential, decreased apoptosis mediator proteins including Bax, Bak, cleaved-caspases, and cleaved-PARP, and increased Bcl-2 and Bcl-xL expression comparing to the UVB-treated control. This finding demonstrated that the PCS extract can reduce the deleterious effects from UVB exposure through decreased intracellular ROS, DNA damage, and apoptosis induction on HaCaT cells. © 2021 Wolters Kluwer Medknow Publications. All rights reserved.

Subject(s)

Caspase
 Histone H2AX
 Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
 Plant extract
 Protein Bak
 Protein Bax
 Protein bcl 2
 Protein bcl xl
 Purple corn silk
 Radioprotective agent
 Reactive oxygen metabolite
 Unclassified drug
 Apoptosis
 Article
 Cell damage
 Cell protection
 Cell survival
 Cell viability
 Comparative study
 Controlled study
 Cytotoxicity
 DNA damage
 Drug effect
 HaCat cell line
 Human
 Human cell
 Immunofluorescence
 Maize
 Mitochondrial membrane potential
 MTT assay
 Polyacrylamide gel electrophoresis
 Priority journal
 Protein expression
 Protein localization
 Radiation exposure
 Radiation protection
 Stigma
 Ultraviolet B radiation
 Western blotting

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URI

https://hdl.handle.net/20.500.14740/7837

Collections

Scopus: Year 1983-2021

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