Publication:
Methanolic extract from sea cucumber, Holothuria scabra, induces apoptosis and suppresses metastasis of PC3 prostate cancer cells modulated by MAPK signaling pathway

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

10177825

DOI

10.4014/jmb.2103.03034

Other identifier(s)

2-s2.0-85109461158

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Microbiology and Biotechnology. Vol 31, No.6 (2021), p.775-783

Suggested Citation

Pranweerapaiboon K., Noonong K., Apisawetakan S., Sobhon P., Chaithirayanon K. Methanolic extract from sea cucumber, Holothuria scabra, induces apoptosis and suppresses metastasis of PC3 prostate cancer cells modulated by MAPK signaling pathway. Journal of Microbiology and Biotechnology. Vol 31, No.6 (2021), p.775-783. doi:10.4014/jmb.2103.03034 Retrieved from: https://hdl.handle.net/20.500.14740/7120

Title

Methanolic extract from sea cucumber, Holothuria scabra, induces apoptosis and suppresses metastasis of PC3 prostate cancer cells modulated by MAPK signaling pathway

Author(s)

Pranweerapaiboon K.
 Noonong K.
 Apisawetakan S.
 Sobhon P.
 Chaithirayanon K.

Abstract

Sea cucumber, Holothuria scabra, is a well-known traditional Asian medicine that has been used for suppressing inflammation, promoting wound healing, and improving immunity. Moreover, previous studies demonstrated that the extract from H. scabra contains many bioactive compounds with potent inhibitory effect on tumor cell survival and progression. However, the effect of the methanolic extract from the body wall of H. scabra (BWMT) on human prostate cancer cells has not yet been investigated. In this study, we aimed to investigate the effects and underlying mechanism of BWMT on prostate cancer cell viability and metastasis. BWMT was obtained by maceration with methanol. The effect of BWMT on cell viability was assessed by MTT and colony formation assays. The intracellular ROS accumulation was evaluated using a DCFH-DA fluorescence probe. Hoechst 33342 staining and Annexin V-FITC/PI staining were used to examine the apoptotic-inducing effect of the extract. A transwell migration assay was performed to determine the anti-metastasis effect. BWMT significantly reduced cell viability and triggered cellular apoptosis by accumulating intracellular ROS resulting in the upregulation of JNK and p38 signaling pathways. In addition, BWMT also inhibited the invasion of PC3 cells by downregulating MMP-2/-9 expression via the ERK pathway. Consequently, our study provides BWMT from H. scabra as a putative therapeutic agent that could be applicable against prostate cancer progression. Copyright © 2021 by The Korean Society for Microbiology and Biotechnology.

Subject(s)

Antineoplastic agent
 Caspase 3
 Gelatinase A
 Gelatinase B
 Holothuria scabra extract
 Methanol
 Mitogen activated protein kinase p38
 Natural product
 Protein Bax
 Protein bcl 2
 Reactive oxygen metabolite
 Unclassified drug
 Antineoplastic agent
 Reactive oxygen metabolite
 Article
 Cancer inhibition
 Cell cycle arrest
 Cell invasion
 Concentration response
 Controlled study
 Holothuria scabra
 Human
 Human cell
 IC50
 JNK signaling
 MAPK signaling
 Metastasis inhibition
 Migration inhibition
 PC-3 [Human prostate carcinoma] cell line
 Proapoptotic activity
 Sea cucumber
 Animal
 Apoptosis
 Cell cycle checkpoint
 Cell motion
 Cell survival
 Chemistry
 Drug effect
 Holothuria
 Isolation and purification
 Male
 Metabolism
 Pathology
 Prostate tumor
 Animals
 Antineoplastic Agents
 Apoptosis
 Cell Cycle Checkpoints
 Cell Movement
 Cell Survival
 Holothuria
 Humans
 Male
 MAP Kinase Signaling System
 Methanol
 PC-3 Cells
 Prostatic Neoplasms
 Reactive Oxygen Species

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URI

https://hdl.handle.net/20.500.14740/7120

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Scopus: Year 1983-2021

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