Publication:
Development of an automated wax-printed paper-based lateral flow device for alpha-fetoprotein enzyme-linked immunosorbent assay

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

9565663

DOI

10.1016/j.bios.2017.10.051

Other identifier(s)

2-s2.0-85032704512

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Biosensors and Bioelectronics. Vol 102, (2018), p.27-32

Suggested Citation

Preechakasedkit P., Siangproh W., Khongchareonporn N., Ngamrojanavanich N., Chailapakul O. Development of an automated wax-printed paper-based lateral flow device for alpha-fetoprotein enzyme-linked immunosorbent assay. Biosensors and Bioelectronics. Vol 102, (2018), p.27-32. doi:10.1016/j.bios.2017.10.051 Retrieved from: https://hdl.handle.net/20.500.14740/5913

Title

Development of an automated wax-printed paper-based lateral flow device for alpha-fetoprotein enzyme-linked immunosorbent assay

Author(s)

Preechakasedkit P.
 Siangproh W.
 Khongchareonporn N.
 Ngamrojanavanich N.
 Chailapakul O.

Abstract

In this study, a novel wax-printed paper-based lateral flow device has been developed as an alternative approach for an automated and one-step enzyme-linked immunosorbent assay (ELISA). The design pattern consisted of a non-delayed channel, a wax-delayed channel, a test zone and a control zone. This system was easily fabricated on a nitrocellulose membrane using a wax-printing method and then baked in an oven at 100 °C for 1 min. The four barriers of the wax-delayed channel could delay the flow time for 11 s compared to the flow time of the non-delayed channel. To use the device under optimal conditions, alpha-fetoprotein (AFP) was detected at a limit of detection of 1 ng mL−1 and assessed with the naked eye within 10 min. A colorimetric intensity was also measured using a smart phone and computer software at a linear range of 0.1–100 ng mL−1 with a good correlation. Furthermore, the proposed device was successfully applied to detect AFP in human serum. Therefore, the wax-printing demonstrates a user-friendly, easy and quick method for the fabrication of the device, which could be used as a one-step, portable, disposable, low-cost, simple, instrument-free and point-of-care device for the automated ELISA. © 2017

Subject(s)

Automation
 Enzymes
 Smartphones
 Alpha fetoprotein
 Enzyme linked immunosorbent assay
 Lateral Flow
 Point-of-care testing
 Wax printings
 Bioassay
 Alpha fetoprotein
 Pyroxylin
 Alpha fetoprotein
 Wax
 Article
 Bioprinting
 Colorimetry
 Concentration (parameters)
 Controlled study
 Enzyme linked immunosorbent assay
 Human
 Limit of detection
 Measurement precision
 Nanofabrication
 Point of care testing
 Wax printed paper based lateral flow device
 Chemistry
 Devices
 Economics
 Enzyme linked immunosorbent assay
 Equipment design
 Evaluation study
 Genetic procedures
 Microfluidic analysis
 Paper
 Smartphone
 Alpha-Fetoproteins
 Biosensing Techniques
 Collodion
 Colorimetry
 Enzyme-Linked Immunosorbent Assay
 Equipment Design
 Humans
 Limit of Detection
 Microfluidic Analytical Techniques
 Paper
 Point-of-Care Testing
 Smartphone
 Waxes

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URI

https://hdl.handle.net/20.500.14740/5913

Collections

Scopus: Year 1983-2021

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