Publication:
Apoptotic induction of skin cancer cell death by plant extracts

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876827175

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S60-S64

Suggested Citation

Thuncharoen W., Chulasiri M., Nilwarangkoon S., Nakamura Y., Watanapokasin R. Apoptotic induction of skin cancer cell death by plant extracts. Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S60-S64. Retrieved from: https://hdl.handle.net/20.500.14740/6763

Title

Apoptotic induction of skin cancer cell death by plant extracts

Author(s)

Thuncharoen W.
 Chulasiri M.
 Nilwarangkoon S.
 Nakamura Y.
 Watanapokasin R.

Abstract

Objective: The aim of the present study was to investigate the effects of plant extracts on cancer apoptotic induction. Material and Method: Human epidermoid carcinoma A431 cell line, obtained from the American Type Culture Collection (ATCC, Manassas, VA), was maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C, 5% carbon dioxide (CO2). Plant extract solutions were obtained from S & J international enterprises public company limited. These plant extracts include 50% hydroglycol extracts from Etlingera elatior (Jack) R.M.Smith (torch ginger; EE), Rosa damascene (damask rose; DR) and Rafflesia kerrii Meijer (bua phut; RM). The cell viability, time and dose dependency were determined by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. A431 cells were treated with the plant extracts and stained with Hoechst 33342 fluorescent staining dye. Results: Cell viability was demonstrated by the inhibitory concentration 50% (IC50). The anti-proliferative effects were shown to be dependent on time and dose. Typical characteristics of apoptosis which are cell morphological changes and chromatin condensation were clearly observed. Conclusion: The plant extracts was shown to be effective for anti-proliferation and induction of apoptosis cell death in skin cancer cells. Therefore, mechanisms underlying the cell death and its potential use for treatment of skin cancer will be further studied.

Subject(s)

DNA
 Etlingera elatior extract
 Ginger extract
 Plant extract
 Rafflesia kerrii meijer extract
 Rosa damascene extract
 Unclassified drug
 Antiproliferative activity
 Apoptosis
 Article
 Cell culture
 Cell proliferation
 Cell viability
 Controlled study
 Cytotoxicity
 Flower
 Human
 Human cell
 IC 50
 Rosa damascena
 Skin cancer
 Apoptosis
 Cell Line, Tumor
 Cell Survival
 Flowers
 Humans
 Plant Extracts
 Rosa
 Skin Neoplasms
 Staining and Labeling

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URI

https://hdl.handle.net/20.500.14740/6763

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Scopus: Year 1983-2021

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