Publication:
PCR-ELISA for diagnosis of Trypanosoma evansi in animals and vector

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

8908508

DOI

10.1006/mcpr.2002.0412

Other identifier(s)

2-s2.0-0036599194

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Molecular and Cellular Probes. Vol 16, No.3 (2002), p.173-177

Suggested Citation

Chansiri K., Khuchareontaworn S., Nopporn S. PCR-ELISA for diagnosis of Trypanosoma evansi in animals and vector. Molecular and Cellular Probes. Vol 16, No.3 (2002), p.173-177. doi:10.1006/mcpr.2002.0412 Retrieved from: https://hdl.handle.net/20.500.14740/6798

Title

PCR-ELISA for diagnosis of Trypanosoma evansi in animals and vector

Author(s)

Chansiri K.
 Khuchareontaworn S.
 Nopporn S.

Abstract

A highly sensitive and specific polymerase chain reaction (PCR) based assay for the detection of Trypanosoma evansi present in the blood of different animals and vector was developed. A simple lysis method was used to remove of the red blood cells to facilitate direct input of samples into the PCR reactions. The primer set was designed and synthesized to amplify a single band of 257 bp PCR product that was subsequently examined by enzyme-linked immunosorbent assay (ELISA). The sensitivity limit of PCR-ELISA was 0.01 pg that was corresponded to 1 parasite/ml of blood. No cross-reactivity of the assay was observed against Babesia boris, B. bigemina, Anaplasma marginale, Theileria sp. and host DNA. The PCR-ELISA was shown to detect 33 samples of T. evansi infected blood of animals and 10 mosquitoes from different geographical area in Thailand. The results were corresponded to those of the PCR and mouse inoculation. This implies that the technique of PCR-ELISA is not only beneficial for diagnosis of the parasite but also useful for epidemiological study and designing rational trypanosomiasis control program. © 2002 Elsevier Science Ltd. All rights reserved.

Subject(s)

DNA
 Protozoal DNA
 Analytic method
 Anaplasma
 Anaplasma marginale
 Article
 Babesia
 Babesia bigemina
 Babesia bovis
 Buffalo
 Cattle
 Controlled study
 Cross reaction
 Deer
 Diagnostic accuracy
 Diagnostic value
 Enzyme linked immunosorbent assay
 Mosquito
 Mouse
 Nonhuman
 Polymerase chain reaction
 Priority journal
 Sensitivity and specificity
 Swine
 Thailand
 Theileria
 Trypanosoma evansi
 Animal
 Animal disease
 Comparative study
 Disease carrier
 Enzyme linked immunosorbent assay
 Evaluation
 Genetics
 Methodology
 Parasitology
 Polymerase chain reaction
 Standard
 Trypanosoma
 Trypanosomiasis
 Anaplasma
 Anaplasma marginale
 Animalia
 Babesia
 Theileria
 Theileria sp.
 Trypanosoma
 Trypanosoma evansi
 Animals
 Buffaloes
 Cattle
 Cross Reactions
 Culicidae
 Deer
 DNA, Protozoan
 Enzyme-Linked Immunosorbent Assay
 Insect Vectors
 Mice
 Polymerase Chain Reaction
 Sensitivity and Specificity
 Trypanosoma
 Trypanosomiasis

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URI

https://hdl.handle.net/20.500.14740/6798

Collections

Scopus: Year 1983-2021

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