Publication:
The expression of Per1 and Aa-nat genes in the pineal gland of postnatal rats

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876894509

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S69-S75

Suggested Citation

Wongchitrat P., Govitrapong P., Phansuwan-Pujito P. The expression of Per1 and Aa-nat genes in the pineal gland of postnatal rats. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S69-S75. Retrieved from: https://hdl.handle.net/20.500.14740/6889

Title

The expression of Per1 and Aa-nat genes in the pineal gland of postnatal rats

Author(s)

Wongchitrat P.
 Govitrapong P.
 Phansuwan-Pujito P.

Abstract

Background: The circadian rhythm of melatonin synthesis is controlled by the master clock, suprachiasmatic nucleus (SCN).The level of melatonin changes throughout the aging process. The SCN's rhythm is driven by autoregulatory feedback loopcomposed of a set of clock genes families and their corresponding proteins. The Period (Per1), one of clock gene developsgradually during postnatal ontogenesis in the rat SCN and is also expressed in the pineal gland.Objective: It is of interest to study the relationship between the postnatal development of Per1 and Aa-nat, genes that producethe rate-limiting enzyme in melatonin synthesis, in the pineal.Material and Method: Daily profiles of mRNA expression of Per1 and Aa-nat were analyzed in the pineal gland of pups atpostnatal ages 4 (P4), P8, P16 and P32, at puberty age of 6 weeks; and in 8 week-old adult rats by real-time PCR.Results: As early as P4, Per1 and Aa-nat mRNAs were expressed and existed at relatively high levels during the nighttime.They gradually increased until puberty and decreased at 8 weeks of age. Additionally, the nocturnal changes of Per1 and Aa-nat mRNA levels in the rat pineal gland from P4 to adults were strongly correlated at r = 0.97 (p < 0.01).Conclusion: The present data indicate that there is a close relationship between the expression pattern of Per1 and that ofmelatonin synthesis during the development of postnatal rats.

Subject(s)

Aralkylamine acetyltransferase
 Melatonin
 Messenger RNA
 PER1 protein
 Article
 Circadian rhythm
 Controlled study
 Gene expression
 Newborn
 Night
 Nonhuman
 Nucleotide sequence
 Pineal body
 Postnatal development
 Puberty
 Rat
 Real time polymerase chain reaction
 Reverse transcription
 Analysis of Variance
 Animals
 Animals, Newborn
 Arylalkylamine N-Acetyltransferase
 Circadian Rhythm
 Gene Expression
 Melatonin
 Nerve Tissue Proteins
 Period Circadian Proteins
 Pineal Gland
 Rats
 Real-Time Polymerase Chain Reaction
 RNA, Messenger
 Suprachiasmatic Nucleus

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URI

https://hdl.handle.net/20.500.14740/6889

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Scopus: Year 1983-2021

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