Publication:
Regulation of electrolyte transport across cultured endometrial epithelial cells by prolactin

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Issued Date

2008

Resource Type

Article

File Type

application/pdf

ISSN

220795

DOI

10.1677/JOE-08-0077

Other identifier(s)

2-s2.0-44949171494

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Endocrinology. Vol 197, No.3 (2008), p.575-582

Suggested Citation

Deachapunya C., Poonyachoti S., Krishnamra N. Regulation of electrolyte transport across cultured endometrial epithelial cells by prolactin. Journal of Endocrinology. Vol 197, No.3 (2008), p.575-582. doi:10.1677/JOE-08-0077 Retrieved from: https://hdl.handle.net/20.500.14740/4006

Title

Regulation of electrolyte transport across cultured endometrial epithelial cells by prolactin

Author(s)

Deachapunya C.
 Poonyachoti S.
 Krishnamra N.

Abstract

The effect of prolactin (PRL) onion transport across the porcine glandular endometrial epithelial cells was studied in primary cell culture using the short-circuit current technique. Addition of 1 μg/ml PRL either to the apical solution or to the basolateral solution produced a peak followed by a sustained increase in Isc, but with a lesser response when PRL was added apically. Basolateral addition of PRL increased the Isc in a concentration-dependent manner with a maximum effiect at 1 μg/ml and an effective concentration value of 120 ng/ml. The PRL-stimulated Isc was significantly reduced by pretreatment with an apical addition of 5-nitro-2-(3-phenylpropylamino) benzoic acid (200 μM), diphenylamine-2-carboxylic acid (1 mM) or 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (200 μM), Cl- channel blockers, but not by amiloride (10 μM), a Na+ channel blocker. In addition, pretreatment with bumetanide (200 μM), a Na + -K + -2Cl- cotransporter inhibitor, in the basolateral solution significantly reduced the PRL-stimulated Isc. Replacement of Cl- or HCO3- in the bathing solutions also decreased the Isc response to PRL. Pretreatment of the monolayer with AG490 (50 μM), an inhibitor of JAK2 activity significantly inhibited the PRL-induced increase in Isc. Western blot analysis of the porcine endometrial epithelial cells revealed the presence of short isoform of PRL receptor (PRLR-S) that could be regulated by 17β-estradiol. The results of this investigation showed that PRL acutely stimulated anion secretion across the porcine endometrial epithelial cells possibly through PRLR-S present in both apical and basolateral membranes. The PRL response appeared to be mediated by the JAK2-dependent pathway. © 2008 Society for Endocrinology.

Subject(s)

4,4' diisothiocyanatostilbene 2,2' disulfonic acid
 5 nitro 2 (3 phenylpropylamino)benzoic acid
 Amiloride
 Bumetanide
 Chloride channel blocking agent
 Efenamic acid
 Estradiol
 Janus kinase 2
 Loop diuretic agent
 N benzyl 2 cyano 3 (3,4 dihydroxyphenyl)acrylamide
 Prolactin
 Prolactin receptor
 Sodium channel blocking agent
 Animal cell
 Animal tissue
 Article
 Cell isolation
 Cell stimulation
 Concentration response
 Controlled study
 Culture medium
 Electrolyte transport
 Endometrium
 Enzyme activity
 Epithelium cell
 Female
 Nonhuman
 Priority journal
 Protein expression
 Short circuit current
 Swine
 Western blotting
 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
 Animals
 Bicarbonates
 Chlorides
 Electrolytes
 Endometrium
 Epithelial Cells
 Female
 Ion Transport
 Janus Kinase 2
 Nitrobenzoates
 Potassium
 Prolactin
 Receptors, Prolactin
 Signal Transduction
 Sodium
 Swine

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URI

https://hdl.handle.net/20.500.14740/4006

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Scopus: Year 1983-2021

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