Publication:
Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

9565663

DOI

10.1016/j.bios.2011.10.031

Other identifier(s)

2-s2.0-84455208100

Rights Holder(s)

Scopus

Bibliographic Citation

Biosensors and Bioelectronics. Vol 31, No.1 (2012), p.562-566

Suggested Citation

Preechakasedkit P., Pinwattana K., Dungchai W., Siangproh W., Chaicumpa W., Tongtawe P., Chailapakul O. Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum. Biosensors and Bioelectronics. Vol 31, No.1 (2012), p.562-566. doi:10.1016/j.bios.2011.10.031 Retrieved from: https://hdl.handle.net/20.500.14740/7114

Title

Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum

Author(s)

Preechakasedkit P.
 Pinwattana K.
 Dungchai W.
 Siangproh W.
 Chaicumpa W.
 Tongtawe P.
 Chailapakul O.

Abstract

An immunochromatographic strip test using gold nanoparticles was developed for the rapid detection of Salmonella typhi (S. typhi) in human serum. The strip test based on the principle of sandwich immunoassay by the specific binding of antigens from S. typhi O901 and antibody of S. typhi O901 on a nitrocellulose membrane. Antibody-gold nanoparticle conjugate was used as the label and was coated onto a glass fiber membrane, which was used as a conjugate pad. To create a test and control zone, antibody of S. typhi O901 and an anti-IgG were dotted on the nitrocellulose membrane, respectively. Positive samples were displayed as red dots at the test and control zones of the nitrocellulose membrane, while negative samples resulted in a red dot only in the control zone. The limit of detection (LOD) was found to be 1.14×10 5cfumL -1, which could be visually detected by the naked eye within 15min. This strip test provided a lower detection limit and analysis time than a dot blot immunoassay (8.88×10 6cfumL -1 for LOD and 110min for reaction time). In addition, our immunochromatographic strip test was employed to detect S. typhi in human serum effectively, with high accuracy. This strip test offers great promise for a rapid, simple and low-cost analysis of S. typhi. © 2011 Elsevier B.V.

Subject(s)

Analysis time
 Control zones
 Fiber membranes
 Gold Nanoparticles
 Human serum
 Immunochromatographic strip test
 Immunochromatographic strips
 Limit of detection
 Lower detection limit
 Naked-eye
 Nanoparticle conjugate
 Nitrocellulose membranes
 Rapid detection
 Salmonella typhi
 Sandwich immunoassay
 Specific binding
 Antibodies
 Antigens
 Body fluids
 Cost accounting
 Glass fibers
 Gold
 Immunology
 Nanoparticles
 Nitrocellulose
 Salmonella
 Testing
 Bacterium antibody
 Glass fiber
 Gold nanoparticle
 Immunoglobulin G antibody
 Pyroxylin
 Antibody labeling
 Antibody specificity
 Antigen binding
 Article
 Bacterium detection
 Blood analysis
 Coated particle
 Controlled study
 Diagnostic accuracy
 Enzyme immunoassay
 Human
 Immunoaffinity chromatography
 Immunoblotting
 Immunochromatographic strip test
 Intermethod comparison
 Limit of detection
 Nonhuman
 Process development
 Reaction time
 Salmonella typhi
 Serum
 Bacterial Load
 Biosensing Techniques
 Blood Chemical Analysis
 Equipment Design
 Equipment Failure Analysis
 Gold
 Humans
 Immunochromatography
 Nanoparticles
 Nanotechnology
 Reagent Strips
 Reproducibility of Results
 Salmonella typhi
 Sensitivity and Specificity
 Accounting
 Antibodies
 Binding
 Cellulose Nitrate
 Glass Fibers
 Gold
 Membranes
 Salmonella typhi

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URI

https://hdl.handle.net/20.500.14740/7114

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Scopus: Year 1983-2021

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