Publication:
HeLa cell transfection using a novel sonoporation system

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

189294

DOI

10.1109/TBME.2010.2089521

Other identifier(s)

2-s2.0-79952934869

Rights Holder(s)

Scopus

Bibliographic Citation

IEEE Transactions on Biomedical Engineering. Vol 58, No.4 (2011), p.927-934

Suggested Citation

Rodamporn S., Harris N.R., Beeby S.P., Boltryk R.J., Sanchez-Elsner T. HeLa cell transfection using a novel sonoporation system. IEEE Transactions on Biomedical Engineering. Vol 58, No.4 (2011), p.927-934. doi:10.1109/TBME.2010.2089521 Retrieved from: https://hdl.handle.net/20.500.14740/7350

Title

HeLa cell transfection using a novel sonoporation system

Author(s)

Rodamporn S.
 Harris N.R.
 Beeby S.P.
 Boltryk R.J.
 Sanchez-Elsner T.

Abstract

Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, p lt; 0.05) at the resonant frequency of 980 kHz at 100 Vp-p (19.5 MPa) with a cell viability of 77% after 10 s of insonication. © 2011 IEEE.

Subject(s)

At resonance
 Cell transfection
 Cell viability
 Chamber design
 HeLa cell
 High rate
 Material thickness
 PZT
 Resonant frequencies
 Sonoporation
 Ultrasonic standing wave
 Ultrasonic standing waves
 Drug delivery
 Elastic waves
 Gene therapy
 Genetic engineering
 Natural frequencies
 Regression analysis
 Transducers
 Ultrasonic waves
 Waves
 Ultrasonics
 Plasmid DNA
 Article
 Cell viability
 Controlled study
 Female
 Genetic transfection
 HeLa cell
 Human
 Human cell
 Human cell culture
 Ionization chamber
 Piezoelectric transducer
 Piezoelectricity
 Plasmid
 Sonoporation
 Transducer
 Ultrasound
 Electroporation
 Equipment Design
 Equipment Failure Analysis
 Hela Cells
 Humans
 Microfluidic Analytical Techniques
 Sonication
 Transfection

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URI

https://hdl.handle.net/20.500.14740/7350

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Scopus: Year 1983-2021

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