Publication: Effect of Vernonia cinerea in improvement of respiratory tissue in chronic nicotine treatment
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Issued Date
2012
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-84876943018
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S47-S55
Suggested Citation
Promputta C., Anupunpisit V., Panyarachun B., Sawatpanich T., Watthanachaiyingcharoen R., Paeratakul O., Kamkaen N., Petpiboolthai H. Effect of Vernonia cinerea in improvement of respiratory tissue in chronic nicotine treatment. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S47-S55. Retrieved from: https://hdl.handle.net/20.500.14740/6880
Abstract
Objective: To demonstrate the effect of Vernonia cinerea (VC) on rat respiratory tissue in chronic nicotine condition. Material and Method: Pathology of rat respiratory tissue was induced by intraperitoneally injection with 1 mg/kg BW of rat. Male Wistar rats were divided into three groups, control group (C), nicotine treated group (N) and nicotine treated with Vernonia cinerea (VC) supplementation (NV, 100 mg/kg BW of rat) for 3 and 6 months. The animals were sacrificed and the respiratory tissues were removed and further processed for paraffin embedment and stained with Hematoxylin & Eosin (H&E), Periodic Acid Schiff (PAS), and Masson's trichrome techniques. Results: The histopathology of lung tissue and trachea occurred in a chronic nicotine treatment. The thickness of alveolar walls and proliferation of alveolar type 2 cell were found. There was remarkable increasing of various inflammatory cells, alveolar macrophages, lymphocytes and plasma cells after nicotine treatment for 6 months. A large number of small blood vessels appeared in the alveolar wall. Nicotine also caused fibrosis which dispersed throughout the lung parenchyma in perivascular, peribronchiole and alveolar wall regions. Moreover, there was the appearance of epithelial cell injury and goblet cell hyperplasia in the trachea. Regarding the VC supplementation, the result of a recovery of alveolar walls, i.e. decreasing of various inflammatory cells and alveolar type 2 cells was clearly demonstrated. In addition, the fibrosis and goblet cell hyperplasia were almost disappeared in the lung tissue after VC treatment. Conclusion: Administration of VC in a chronic nicotine treatment resulted in an improvement of respiratory tissue. The recovery of the respiratory tract, especially trachea and lung tissue was characterized by the remarkable decrease of various inflammatory cells, fibrotic areas, and goblet cell hyperplasia. The VC, therefore shows the potential effect to be a new herbal therapeutic agent for alleviate the symptoms of the respiratory tract caused by nicotine from heavy cigarette smoke.
Subject(s)
Cotinine
Cyanthillium cinereum extract
Nicotine
Plant extract
Unclassified drug
Animal experiment
Animal model
Animal tissue
Article
Cell damage
Cell hyperplasia
Cell infiltration
Cell proliferation
Controlled study
Fibrosis
Goblet cell
Histopathology
Inflammation
Lung alveolus cell type 2
Lung injury
Lung parenchyma
Male
Mass fragmentography
Nonhuman
Rat
Analysis of Variance
Animals
Lung Diseases
Male
Nicotine
Plant Extracts
Rats
Rats, Wistar
Respiratory System
Staining and Labeling
Vernonia
Cyanthillium cinereum extract
Nicotine
Plant extract
Unclassified drug
Animal experiment
Animal model
Animal tissue
Article
Cell damage
Cell hyperplasia
Cell infiltration
Cell proliferation
Controlled study
Fibrosis
Goblet cell
Histopathology
Inflammation
Lung alveolus cell type 2
Lung injury
Lung parenchyma
Male
Mass fragmentography
Nonhuman
Rat
Analysis of Variance
Animals
Lung Diseases
Male
Nicotine
Plant Extracts
Rats
Rats, Wistar
Respiratory System
Staining and Labeling
Vernonia