Publication:
IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria

0
0

Issued Date

2009

Resource Type

Article

File Type

application/pdf

ISSN

14752875

DOI

10.1186/1475-2875-8-286

Other identifier(s)

2-s2.0-74549211889

Rights Holder(s)

Scopus

Bibliographic Citation

Malaria Journal. Vol 8, No.1 (2009)

Suggested Citation

Tangteerawatana P., Perlmann H., Hayano M., Kalambaheti T., Troye-Blomberg M., Khusmith S. IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria. Malaria Journal. Vol 8, No.1 (2009). doi:10.1186/1475-2875-8-286 Retrieved from: https://hdl.handle.net/20.500.14740/3684

Title

IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria

Author(s)

Tangteerawatana P.
 Perlmann H.
 Hayano M.
 Kalambaheti T.
 Troye-Blomberg M.
 Khusmith S.

Abstract

Abstract. Background. The IL4-590 gene polymorphism has been shown to be associated with elevated levels of anti-Plasmodium falciparum IgG antibodies and parasite intensity in the malaria protected Fulani of West Africa. This study aimed to investigate the possible impact of IL4-590C/T polymorphism on anti-P. falciparum IgG subclasses and IgE antibodies levels and the alteration of malaria severity in complicated and uncomplicated malaria patients with or without previous malaria experiences. Methods. Anti-P.falciparum IgG subclasses and IgE antibodies in plasma of complicated and uncomplicated malaria patients with or without previous malaria experiences were analysed using ELISA. IL4-590 polymorphisms were genotyped using RFLP-PCR. Statistical analyses of the IgG subclass levels were done by Oneway ANOVA. Genotype differences were tested by Chi-squared test. Results. The IL4-590T allele was significantly associated with anti-P. falciparum IgG3 antibody levels in patients with complicated (P = 0.031), but not with uncomplicated malaria (P = 0.622). Complicated malaria patients with previous malaria experiences carrying IL4-590TT genotype had significantly lower levels of anti-P. falciparum IgG3 (P = 0.0156), while uncomplicated malaria patients with previous malaria experiences carrying the same genotype had significantly higher levels (P = 0.0206) compared to their IL4-590 counterparts. The different anti-P. falciparum IgG1 and IgG3 levels among IL4 genotypes were observed. Complicated malaria patients with previous malaria experiences tended to have lower IgG3 levels in individuals carrying TT when compared to CT genotypes (P = 0.075). In contrast, complicated malaria patients without previous malaria experiences carrying CC genotype had significantly higher anti-P. falciparum IgG1 than those carrying either CT or TT genotypes (P = 0.004, P = 0.002, respectively). Conclusion. The results suggest that IL4-590C or T alleles participated differently in the regulation of anti-malarial antibody isotype profiles in primary and secondary malaria infection and, therefore, could play an important role in alteration of malaria severity. © 2009 Tangteerawatana et al; licensee BioMed Central Ltd.

Subject(s)

Immunoglobulin E antibody
 Immunoglobulin G antibody
 Interleukin 4
 Immunoglobulin class
 Immunoglobulin E
 Immunoglobulin G
 Interleukin 4
 Adolescent
 Adult
 Aged
 Article
 Controlled study
 Disease severity
 Disease transmission
 Endemic disease
 Enzyme linked immunosorbent assay
 Female
 Genetic variability
 Genotype
 Human
 Major clinical study
 Malaria
 Male
 Plasmodium falciparum
 Restriction fragment length polymorphism
 Single nucleotide polymorphism
 Antibody production
 Blood
 Genetic variability
 Genetics
 Hospitalization
 Immunology
 Malaria falciparum
 Middle aged
 Parasitology
 Polymerase chain reaction
 Adolescent
 Adult
 Aged
 Antibody Formation
 Enzyme-Linked Immunosorbent Assay
 Female
 Genetic Variation
 Genotype
 Humans
 Immunoglobulin E
 Immunoglobulin G
 Immunoglobulin Isotypes
 Interleukin-4
 Malaria, Falciparum
 Male
 Middle Aged
 Plasmodium falciparum
 Polymerase Chain Reaction
 Polymorphism, Restriction Fragment Length
 Severity of Illness Index
 Young Adult

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/3684

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By