Publication:
Hepatoprotective Effect of Probiotic Lactic Acid Bacteria on Thioacetamide-Induced Liver Fibrosis in Rats

3
0

Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

18671306

DOI

10.1007/s12602-020-09663-6

Other identifier(s)

2-s2.0-85085689121

Rights Holder(s)

Scopus

Bibliographic Citation

Probiotics and Antimicrobial Proteins. Vol 13, No.1 (2021), p.40-50

Suggested Citation

Jantararussamee C., Rodniem S., Taweechotipatr M., Showpittapornchai U., Pradidarcheep W. Hepatoprotective Effect of Probiotic Lactic Acid Bacteria on Thioacetamide-Induced Liver Fibrosis in Rats. Probiotics and Antimicrobial Proteins. Vol 13, No.1 (2021), p.40-50. doi:10.1007/s12602-020-09663-6 Retrieved from: https://hdl.handle.net/20.500.14740/7893

Title

Hepatoprotective Effect of Probiotic Lactic Acid Bacteria on Thioacetamide-Induced Liver Fibrosis in Rats

Author(s)

Jantararussamee C.
 Rodniem S.
 Taweechotipatr M.
 Showpittapornchai U.
 Pradidarcheep W.

Abstract

Hepatic fibrosis is a reversible wound-healing response characterized by the accumulation of extracellular matrix. Probiotics have been used to prevent and treat various disorders. The aim of the present study was to investigate the hepatoprotective effects of probiotic lactic acid bacteria (mixture of Lactobacillus paracasei, Lactobacillus casei, and Weissella confusa) on thioacetamide (TAA)–induced liver fibrosis in rats. Thirty-five male Wistar rats were randomly divided into five groups: (1) control, (2) TAA, (3) TAA+probiotics, (4) TAA+silymarin, and (5) probiotics. Group 1 rats received a standard diet. In groups 2–4, fibrosis was induced by intraperitoneal injection of TAA (200 mg/kg BW) 3 times weekly for 8 consecutive weeks. Group 4 received TAA plus 100 mg/kg BW of silymarin 2 times weekly. Groups 3 and 5 were fed 109 CFU/mL viable microbial cells daily by gavage. The rats were sacrificed after 8 weeks of treatment. Liver tissues were collected immediately and processed for histopathological, lipid peroxidation, and Western blot analyses of TNF-α, TGF-β1, and α-SMA. Blood serum was collected to measure liver enzymes. Rats in the TAA groups suffered from hepatic injury (increased serum enzyme levels, liver inflammation, and increased concentration of TNF-α, TGF-β1, and α-SMA proteins) and extensive liver fibrosis. In contrast, TAA-treated rats receiving probiotics or silymarin had significantly lower serum enzyme levels, less inflammation, and less fibrosis. Liver damage was lower in the TAA+probiotics-treated group. Consumption of a mixture of probiotic lactic acid bacteria attenuates the development of liver fibrosis. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.

Subject(s)

Alanine aminotransferase
 Alkaline phosphatase
 Alpha smooth muscle actin
 Aspartate aminotransferase
 Malonaldehyde
 Probiotic agent
 Silymarin
 Thioacetamide
 Transforming growth factor beta1
 Tumor necrosis factor
 Thioacetamide
 Animal experiment
 Animal model
 Animal tissue
 Article
 Body weight
 Cell infiltration
 Controlled study
 Hepatitis
 Histopathology
 Lactobacillus casei
 Lactobacillus paracasei
 Lipid peroxidation assay
 Liver fibrosis
 Liver injury
 Liver necrosis
 Liver protection
 Liver weight
 Male
 Nonhuman
 Oxidative stress
 Polyacrylamide gel electrophoresis
 Protein expression
 Rat
 Small intestine
 Weissella
 Weissella confusa
 Western blotting
 Animal
 Lactobacillales
 Liver cirrhosis
 Metabolism
 Pathology
 Wistar rat
 Animals
 Lactobacillales
 Liver Cirrhosis
 Male
 Rats
 Rats, Wistar
 Thioacetamide

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URI

https://hdl.handle.net/20.500.14740/7893

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Scopus: Year 1983-2021

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