Publication:
Melatonin increases proliferation of cultured neural stem cells obtained from adult mouse subventricular zone

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

7423098

DOI

10.1111/j.1600-079X.2010.00794.x

Other identifier(s)

2-s2.0-77956299657

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Pineal Research. Vol 49, No.3 (2010), p.291-300

Suggested Citation

Sotthibundhu A., Phansuwan-Pujito P., Govitrapong P. Melatonin increases proliferation of cultured neural stem cells obtained from adult mouse subventricular zone. Journal of Pineal Research. Vol 49, No.3 (2010), p.291-300. doi:10.1111/j.1600-079X.2010.00794.x Retrieved from: https://hdl.handle.net/20.500.14740/7543

Title

Melatonin increases proliferation of cultured neural stem cells obtained from adult mouse subventricular zone

Author(s)

Sotthibundhu A.
 Phansuwan-Pujito P.
 Govitrapong P.

Abstract

Melatonin, a circadian rhythm-promoting molecule secreted mainly by the pineal gland, has a variety of biological functions and neuroprotective effects including control of sleep-wake cycle, seasonal reproduction, and body temperature as well as preventing neuronal cell death induced by neurotoxic substances. Melatonin also modulates neural stem cell (NSC) function including proliferation and differentiation in embryonic brain tissue. However, the involvement of melatonin in adult neurogenesis is still not clear. Here, we report that precursor cells from adult mouse subventricular zone (SVZ) of the lateral ventricle, the main neurogenic area of the adult brain, express melatonin receptors. In addition, precursor cells derived from this area treated with melatonin exhibited increased proliferative activity. However, when cells were treated with luzindole, a competitive inhibitor of melatonin receptors, or pertussis toxin, an uncoupler of Gi from adenylate cyclase, melatonin-induced proliferation was reduced. Under these conditions, melatonin induced the differentiation of precursor cells to neuronal cells without an upregulation of the number of glia cells. Because stem cell replacement is thought to play an important therapeutic role in neurodegenerative diseases, melatonin might be beneficial for stimulating endogenous neural stem cells. © 2010 John Wiley & Sons A/S.

Subject(s)

Adenylate cyclase
 Luzindole
 Melatonin
 Melatonin receptor
 Pertussis toxin
 Animal cell
 Article
 Cell culture
 Cell differentiation
 Cell proliferation
 Concentration response
 Controlled study
 Glia cell
 Mouse
 Nerve degeneration
 Nervous system development
 Neural stem cell
 Nonhuman
 Stem cell transplantation
 Subventricular zone
 Upregulation
 Analysis of Variance
 Animals
 Antioxidants
 Blotting, Western
 Cell Proliferation
 Cells, Cultured
 Immunohistochemistry
 Lateral Ventricles
 Melatonin
 Mice
 Neural Stem Cells
 Receptors, Melatonin
 Tryptamines

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URI

https://hdl.handle.net/20.500.14740/7543

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Scopus: Year 1983-2021

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