Publication:
Ethanolic extract of thevetia peruviana flowers enhances TNF-α and TRAIL-induced apoptosis of human cervical cancer cells via intrinsic and extrinsic pathways

0
0

Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

17921074

DOI

10.3892/ol.2017.5748

Other identifier(s)

2-s2.0-85015321143

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Oncology Letters. Vol 13, No.4 (2017), p.2791-2798

Suggested Citation

Managit C., Sakurai H., Saiki I. Ethanolic extract of thevetia peruviana flowers enhances TNF-α and TRAIL-induced apoptosis of human cervical cancer cells via intrinsic and extrinsic pathways. Oncology Letters. Vol 13, No.4 (2017), p.2791-2798. doi:10.3892/ol.2017.5748 Retrieved from: https://hdl.handle.net/20.500.14740/4200

Title

Ethanolic extract of thevetia peruviana flowers enhances TNF-α and TRAIL-induced apoptosis of human cervical cancer cells via intrinsic and extrinsic pathways

Author(s)

Managit C.
 Sakurai H.
 Saiki I.

Abstract

Tumor necrosis factor-α (TNF-α) and TNF-related apoptosis-inducing ligand (TRAIL) are promising candidates for cancer treatment due to their ability to induce apoptosis through death receptor stimulation. However, their usage may be limited due to the resistance of cancer cells to TNF-α- and TRAIL-induced apoptosis. Currently, there is interest in screening for natural products that can sensitize cancer cells to TNF-α- and TRAIL-induced apoptosis for their use in combination with TNF-α or TRAIL. It was previously reported that the bark extract of Thevetia peruviana showed a reversal effect on TRAIL-resistance in human gastric adenocarcinoma cell lines. In the present study, the effects of the ethanolic extract of T. peruviana flowers on TNF-α- and TRAIL-induced apoptosis of human cervical cancer HeLa cells were investigated in vitro by determining cell viability and apoptosis using a WST-1 cell proliferation assay and immunoblot analysis, respectively. The ethanolic extract of T. peruviana flowers promoted TNF-α and TRAIL-mediated cell death through the activation of the caspase cascade, poly(ADP-ribose) polymerase and BH3-interacting domain death agonist cleavage. Combined treatment using the extract plus TNF-α resulted in downregulation of anti-apoptotic protein, including myeloid cell leukemia sequence-1, B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis protein and survivin, while the combined treatment with TRAIL downregulated Bcl-XL. Thus, the ethanolic extract of T. peruviana flowers has potential in sensitizing the TNF-α- and TRAIL-induced apoptosis of HeLa cells via the intrinsic and extrinsic pathways. © 2017, Spandidos Publications. All rights reserved.

Subject(s)

Alcohol
 Cardiac glycoside
 Caspase
 Death receptor
 Inhibitor of apoptosis protein
 Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
 Plant extract
 Protein bcl xl
 Protein mcl 1
 Survivin
 Thevetia peruviana extract
 Tumor necrosis factor
 Tumor necrosis factor related apoptosis inducing ligand
 Unclassified drug
 X linked inhibitor of apoptosis
 Adenocarcinoma cell line
 Apoptosis
 Article
 B cell lymphoma
 Bark
 Cell proliferation
 Cell proliferation assay
 Cell viability
 Controlled study
 Down regulation
 Electrophoresis
 Flower
 Human
 Human cell
 Immunoblotting
 Stomach adenocarcinoma
 Uterine cervix cancer
 Western blotting

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/4200

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By