Publication: Degradation of poly(Butylene succinate) and poly(butylene succinate)/poly(lactide) blends using serine protease produced from laceyella sacchari LP175
1
0
Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
27740226
Other identifier(s)
2-s2.0-85121641152
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Trends in Sciences. Vol 18, No.20 (2021)
Suggested Citation
Samaimai S., Krajangsang S., Kitpreechavanich V., Borthong J., Lomthong T. Degradation of poly(Butylene succinate) and poly(butylene succinate)/poly(lactide) blends using serine protease produced from laceyella sacchari LP175. Trends in Sciences. Vol 18, No.20 (2021). doi:10.48048/tis.2021.37 Retrieved from: https://hdl.handle.net/20.500.14740/8155
Abstract
The thermophilic filamentous bacterium Laceyella sacchari LP175 was cultivated in a 10.0 L airlift fermenter to produce serine protease at 50 °C. Maximal serine protease activity at 1,123.32 ± 15.8 U/mL was obtained for cultivation at 0.6 vvm aeration rate for 36 h. The crude enzyme was applied for degradation of poly (butylene succinate) (PBS), and poly (butylene succinate)/poly(lactide) blend (PBS/PLA) powders at 50 °C for 48 h with different substrates and enzyme concentrations. Results showed that serine protease produced from L. sacchari LP175 degraded PBS and PBS/PLA at 46.5 ± 2.05 and 49.8 ± 1.45 %, respectively, at an initial substrate concentration of 100 g/L with 1,200 U/mL of serine protease activity. Percentage degradation of PBS and PBS/PLA was improved to 51.4 ± 1.06 and 56.9 ± 1.42 %, respectively, when upscaled in a 2.0 L stirrer fermenter with 200 rpm agitation rate. Degradation products evaluated by a scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that serine protease produced from L. sacchari LP175 degraded both PBS and PBS/PLA polymers. Results showed that microbial enzyme technology could be used to degrade PBS and PBS/PLA blend polymers and reduce the accumulation of waste. © 2021, Walailak University. All rights reserved.