Publication: Effect of Basic Fibroblast Growth Factor on Expression of Let-7 MicroRNA in Proliferation of Human Dental Pulp Cells
0
0
Issued Date
2022
Resource Type
Language
eng
File Type
application/pdf
ISSN
25869981
Rights Holder(s)
Scopus
Bibliographic Citation
Oman Medical Journal. Vol 37, No.6 (2022)
Suggested Citation
Jirawechwongsakul P., Taebunpakul P., Pavasant P. Effect of Basic Fibroblast Growth Factor on Expression of Let-7 MicroRNA in Proliferation of Human Dental Pulp Cells. Oman Medical Journal. Vol 37, No.6 (2022). doi:10.31584/jhsmr.2022876 Retrieved from: https://hdl.handle.net/20.500.14740/9177
Author(s)
Abstract
Objective: Basic fibroblast growth factor (bFGF) plays a pivotal role in cell proliferation, differentiation and extracellular matrix turnover in various tissues. In human dental pulp cells (HDPCs), let-7 microRNA is involved in cell proliferation and differentiation. There is little information on the effect of bFGF-induced cell proliferation on let-7 microRNA in HDPCs. This study investigated the effect of bFGF on let-7g, let-7f, and let-7i microRNAs and some of the genes involved in cell proliferation including p53 and Ki67 in HDPCs. Material and Methods: HDPCs were cultured and treated with bFGF at 0, 1, and 5 ng/mL. Cell proliferation was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay at 24 and 48 hours. Additionally, gene expressions of let-7g, let-7f, let-7i microRNAs, and p53 and Ki67 were examined by quantitative real-time polymerase chain reaction at 24 hours. All experiments were performed in triplicate. Results: The results showed that let-7g, let-7f, and let-7i microRNAs were expressed in HDPCs. MTT assays showed that bFGF induced greater cell proliferation than the controls at 24 and 48 hours (p-value<0.050). HDPCs treated with bFGF showed a decrease in p53 expression (p-value<0.001) while Ki67 expression increased (p-value<0.001). The expression of let-7g microRNA decreased under the influence of bFGF (p-value<0.050). However, bFGF had no effect on expression of let-7f and let-7i microRNAs (p-value>0.050). Conclusion: Our preliminary study showed that exogenous bFGF could decrease let-7g microRNA expression suggesting that let-7g microRNA may be involved in bFGF-induced HDPCs proliferation. © 2022 JHSMR. Hosting by Prince of Songkla University. All rights reserved.