Publication:
Seven novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant tiger prawn Penaeus monodon

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

10964959

DOI

10.1016/S1096-4959(01)00499-7

Other identifier(s)

2-s2.0-0036185834

Rights Holder(s)

Scopus

Bibliographic Citation

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. Vol 131, No.3 (2002), p.325-337

Suggested Citation

Sithigorngul P., Pupuem J., Krungkasem C., Longyant S., Chaivisuthangkura P., Sithigorngul W., Petsom A. Seven novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant tiger prawn Penaeus monodon. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. Vol 131, No.3 (2002), p.325-337. doi:10.1016/S1096-4959(01)00499-7 Retrieved from: https://hdl.handle.net/20.500.14740/6818

Title

Seven novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant tiger prawn Penaeus monodon

Author(s)

Sithigorngul P.
 Pupuem J.
 Krungkasem C.
 Longyant S.
 Chaivisuthangkura P.
 Sithigorngul W.
 Petsom A.

Abstract

FMRFamide-like immunoreactivity (FLI) was localized in the eyestalk of Penaeus monodon by immunohistochemistry using a combination of three anti-FMRFamide-like peptide (FLPs) monoclonal antibodies. Approximately 3000 small neuronal cell bodies in the lamina ganglionalis; 100 medium to large size at the ganglion between the medulla interna and the medulla terminalis; and 250 medium size around the medulla terminalis were stained intensely. The neuronal processes in neuropils of the medulla externa, medulla interna, medulla terminalis, sinus gland and some nerve fibers in the optic nerve were also recognized. The small cell bodies, approximately 1500 cells, anterior to the medulla externa were stained inconsistently and the neuronal processes were not observed from these cells. Isolation of FLPs from 9000 eyestalks was performed using methanol/acetic/water (90:1:9) extraction. After the extract was partially purified using C18 cartridges, it was further purified by five to seven steps of RP-HPLC using three kinds of columns: C18; C8; and cyano, and three solvent systems: acetonitrile/trifluoro acetic acid; aceonitrile/heptafluoro butyric acid; and acetonitrile/triethyl ammonium acetate. Dot-ELISA using the combination of the same antibodies was used to monitor FLPs in the fractions during purification processes. Seven new sequences of FLPs were identified which can be divided into four subgroups according to the primary structure of the C-terminus: (1) GDRNFLRFamide; (2) AYSNLNYLRFamide; (3) AQPSMRLRFamide, SQPSMRLRFamide, SMPSLRLRFamide and DGRTPALRLRFamide; and (4) GYRKPPFNGSIFamide. These data indicate the high complexity of this peptide family in which multiple forms are usually exist. © 2002 Elsevier Science Inc. All rights reserved.

Subject(s)

Acetic acid
 Acetonitrile
 Alanylglutaminylprolylserylmethionylarginylleucylarginylphenylalaninamide
 Alanyltyrosylserylasparaginylleucylasparaginyltyrosylleucylarginylphenylala ninamide
 Ammonium acetate
 Aspartylglycylarginylthreonylprolylalanylleucylarginylleucylarginylphenylal aninamide
 Butyric acid
 Glycylaspartylarginylasparaginylphenylalanylleucylarginylphenylalaninamide
 Glycyltyrosylarginyllysylprolylprolylphenylalanylasparaginylglycylserylisol eucylphenylalaninamide
 Methanol
 Monoclonal antibody
 Neuropeptide
 Peptide derivative
 Phenylalanylmethionylarginylphenylalaninamide
 Serylglutaminylprolylserylmethionylarginylleucylarginylphenylalaninamide
 Serylmethionylprolylserylleucylarginylleucylarginylphenylalaninamide
 Unclassified drug
 Water
 Amino acid sequence
 Article
 Carboxy terminal sequence
 Controlled study
 Culture medium
 Enzyme linked immunosorbent assay
 Extraction
 Ganglion
 Immunohistochemistry
 Immunoreactivity
 Nerve fiber
 Neuropil
 Nonhuman
 Optic nerve
 Perikaryon
 Priority journal
 Purification
 Reversed phase high performance liquid chromatography
 Staining
 Monodon
 Panthera tigris
 Penaeus monodon

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URI

https://hdl.handle.net/20.500.14740/6818

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Scopus: Year 1983-2021

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