Publication:
Genetic Diversity of Major Piroplasm Surface Protein Genes and Their Allelic Variants of Theileria Parasites in Thai Cattle

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Issued Date

1999

Resource Type

Article

File Type

application/pdf

ISSN

7653697

DOI

10.1292/jvms.61.991

Other identifier(s)

2-s2.0-0033192116

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Veterinary Medical Science. Vol 61, No.9 (1999), p.991-994

Suggested Citation

Sarataphan N., Nilwarangkoon S., Tananyutthawongese C., Kakuda T., Onuma M., Chansiri K. Genetic Diversity of Major Piroplasm Surface Protein Genes and Their Allelic Variants of Theileria Parasites in Thai Cattle. Journal of Veterinary Medical Science. Vol 61, No.9 (1999), p.991-994. doi:10.1292/jvms.61.991 Retrieved from: https://hdl.handle.net/20.500.14740/7181

Title

Genetic Diversity of Major Piroplasm Surface Protein Genes and Their Allelic Variants of Theileria Parasites in Thai Cattle

Author(s)

Sarataphan N.
 Nilwarangkoon S.
 Tananyutthawongese C.
 Kakuda T.
 Onuma M.
 Chansiri K.

Abstract

Twenty-eight field isolated Theileria parasite DNAs obtained from dairy and beef cattle in distinct geographical areas of Thailand were characterized by using polymerase chain reaction (PCR) amplification with six sets of oligonucleotide primers. Three sets of them were modified from two genes of immunodominant major piroplasm surface protein (MPSP) coding for 32 kDa (p32) of T. sergenti and 33/34 kDa (p33/34) of T. buffeli, and MPSP of Theileria spp.(Thai-isolate). The other three sets of primers were basically generated from three alleles of MPSP which were specific for Japanese T. sergenti-Ikeda stock (I-type), Japanese T. sergenti-Chitose stock (C-type) and Australian T. buffeli-Warwick stock (B1-type), respectively. The results indicated that 14 out of 28 isolates were amplified by the Thai-specific primer whereas 6 isolates were amplified by the p32 specific primer and the other 5 isolates were amplified by the p32 and Thai-specific primers. In addition, by using the allele-specific PCR, 14 out of 28 isolates contained C-type MPSP whereas 3 isolates contained B1 type parasites. Interestingly, 20 out of 28 isolates could be amplified by the Thai-specific primer. The majority of Theileria parasites distributed in Thailand contained Thai type parasites, whereas C-type parasites showed the mixed population with B1 and Thai type parasites. No I type parasite was detected.

Subject(s)

Bos taurus
 Ikeda
 Theileria
 Membrane protein
 Primer DNA
 Protozoal DNA
 Protozoal protein
 Agar gel electrophoresis
 Allele
 Animal
 Animal disease
 Animal parasitosis
 Article
 Cattle
 Chemistry
 Classification
 Female
 Genetic variability
 Genetics
 Molecular weight
 Parasitology
 Polymerase chain reaction
 Thailand
 Theileria
 Alleles
 Animals
 Cattle
 DNA Primers
 DNA, Protozoan
 Electrophoresis, Agar Gel
 Female
 Membrane Proteins
 Molecular Weight
 Polymerase Chain Reaction
 Protozoan Proteins
 Thailand
 Theileria
 Theileriasis
 Variation (Genetics)

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URI

https://hdl.handle.net/20.500.14740/7181

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Scopus: Year 1983-2021

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