Publication:
Modulated expression of the HIV-1 2LTR zinc finger efficiently interferes with the HIV integration process

0
0

Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

1448463

DOI

10.1042/BSR20181109

Other identifier(s)

2-s2.0-85052957177

Rights Holder(s)

Scopus

Bibliographic Citation

Bioscience Reports. Vol 38, No.5 (2018)

Suggested Citation

Moonmuang S., Saoin S., Chupradit K., Sakkhachornphop S., Israsena N., Rungsiwiwut R., Tayapiwatana C. Modulated expression of the HIV-1 2LTR zinc finger efficiently interferes with the HIV integration process. Bioscience Reports. Vol 38, No.5 (2018). doi:10.1042/BSR20181109 Retrieved from: https://hdl.handle.net/20.500.14740/5852

Title

Modulated expression of the HIV-1 2LTR zinc finger efficiently interferes with the HIV integration process

Author(s)

Moonmuang S.
 Saoin S.
 Chupradit K.
 Sakkhachornphop S.
 Israsena N.
 Rungsiwiwut R.
 Tayapiwatana C.

Abstract

Lentiviral vectors have emerged as the most efficient system to stably transfer and insert genes into cells. By adding a tetracycline (Tet)-inducible promoter, transgene expression delivered by a lentiviral vector can be expressed whenever needed and halted when necessary. Here we have constructed a doxycycline (Dox)-inducible lentiviral vector which efficiently introduces a designed zinc finger protein, 2-long terminal repeat zinc-finger protein (2LTRZFP), into hematopoietic cell lines and evaluated its expression in pluripotent stem cells. As a result this lentiviral inducible system can regulate 2LTRZFP expression in the SupT1 T-cell line and in pluripotent stem cells. Using this vector, no basal expression was detected in the T-cell line and its induction was achieved with low Dox concentrations. Remarkably, the intracellular regulatory expression of 2LTRZFP significantly inhibited HIV-1 integration and replication in HIV-inoculated SupT1 cells. This approach could provide a potential tool for gene therapy applications, which efficiently control and reduce the side effect of therapeutic genes expression. © 2018 The Author(s).

Subject(s)

Doxycycline
 Lentivirus vector
 Protein 2LTR
 Tetracycline
 Unclassified drug
 Zinc finger protein
 Doxycycline
 Tetracycline
 Zinc finger protein
 Antiviral activity
 Article
 Controlled study
 Gene expression
 Gene therapy
 Hematopoietic cell line
 Human immunodeficiency virus 1 infection
 Nonhuman
 Pluripotent stem cell
 Protein expression
 Protein stability
 SUP-T1 cell line
 T lymphocyte
 Therapy effect
 Virus DNA cell DNA interaction
 Virus induction
 Virus interference
 Virus replication
 Dose response
 Drug effect
 Gene expression regulation
 Gene vector
 Genetics
 HEK293 cell line
 Human
 Human immunodeficiency virus 1
 Human immunodeficiency virus infection
 Lentivirus
 Long terminal repeat
 Pathogenicity
 Physiology
 Procedures
 Transgene
 Virology
 Virus DNA cell DNA interaction
 Dose-Response Relationship, Drug
 Doxycycline
 Gene Expression Regulation
 Genetic Therapy
 Genetic Vectors
 HEK293 Cells
 HIV Infections
 HIV Long Terminal Repeat
 HIV-1
 Humans
 Lentivirus
 Pluripotent Stem Cells
 Tetracycline
 Transgenes
 Virus Integration
 Zinc Fingers

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/5852

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By