Publication:
Authentication of Coscinium fenestratum among the other Menispermaceae plants prescribed in Thai folk medicines

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

9186158

DOI

10.1248/bpb.33.91

Other identifier(s)

2-s2.0-75149167072

Rights Holder(s)

Scopus

Bibliographic Citation

Biological and Pharmaceutical Bulletin. Vol 33, No.1 (2010), p.91-94

Suggested Citation

Watthanachaiyingcharoen R., Komatsu K., Zhu S., Vajragupta O., Leelamanit W. Authentication of Coscinium fenestratum among the other Menispermaceae plants prescribed in Thai folk medicines. Biological and Pharmaceutical Bulletin. Vol 33, No.1 (2010), p.91-94. doi:10.1248/bpb.33.91 Retrieved from: https://hdl.handle.net/20.500.14740/7668

Title

Authentication of Coscinium fenestratum among the other Menispermaceae plants prescribed in Thai folk medicines

Author(s)

Watthanachaiyingcharoen R.
 Komatsu K.
 Zhu S.
 Vajragupta O.
 Leelamanit W.

Abstract

In Ayurveda and Thai traditional medicines, material from Coscinium fenestratum is commonly prescribed as active ingredients with diverse therapeutic purposes. However, C. fenestratum is also a seriously endangered medicinal liana. Thus, its crude material is very rare and is being substituted with substances from Arcangelisia flava or Fibraurea tinctoria (Menispermaceae), which have high morphological similarity. In this current study, nuclear 18S ribosomal RNA (rRNA) gene and nuclear ribosomal DNA internal transcribed spacer (ITS) gene sequences with the polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique were exploited to identify these three species. The nuclear 18S rRNA gene sequences of C. fenestratum, A. flava, and F. tinctoria consisted of 1809, 1805, and 1809 base pairs (bps), respectively, while their ITS gene regions were 694, 622, and 631 bps in length, respectively. The 18S rRNA gene of C. fenestratum digested with SmaI restriction enzyme displayed the electrophoresis profile of 729 and 790 bps; for A. flava and F. tinctoria, the digested products showed fragments of 1519 bps. Although the ITS gene regions of A. flava and F. tinctoria had unrecognized sequences with SalI, the SalI-digested ITS of C. fenestratum exhibited fragments of approximately 599 bp. Thus, the 18S rRNA gene and ITS gene sequences with PCR-RFLPs were proven to be powerful molecular markers for identifying C. fenestratum and distinguishing it from the other two Menispermaceae plants. © 2010 Pharmaceutical Society of Japan.

Subject(s)

Internal transcribed spacer
 Ribosome DNA
 Ribosome RNA
 Arcangelisia flava
 Article
 Controlled study
 Coscinium fenestratum
 Electrophoresis
 Fibraurea tinctoria
 Gene sequence
 Menispermaceae
 Nonhuman
 Nucleotide sequence
 Polymerase chain reaction
 Restriction fragment length polymorphism
 Traditional medicine
 Base Sequence
 DNA, Ribosomal Spacer
 Drug Contamination
 Electrophoresis
 Medicine, East Asian Traditional
 Menispermaceae
 Plant Components, Aerial
 Polymerase Chain Reaction
 Polymorphism, Restriction Fragment Length
 RNA, Ribosomal, 18S
 Sequence Analysis, DNA

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URI

https://hdl.handle.net/20.500.14740/7668

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Scopus: Year 1983-2021

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