Publication: Synergistic Antifungal Properties, Chemical Composition, and Frontier Molecular Orbital Analysis of Essential Oils from Lemongrass, Kaffir Lime, Lime, Dill, and Shatavari Against Malassezia furfur
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Issued Date
2025-06-01
Resource Type
ISSN
16616596
eISSN
14220067
Scopus ID
2-s2.0-105008998992
Journal Title
International Journal of Molecular Sciences
Volume
26
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences Vol.26 No.12 (2025)
Suggested Citation
Tadtong S., Chantavacharakorn R., Khayankan S., Akachaipaibul P., Eiamart W., Samee W. Synergistic Antifungal Properties, Chemical Composition, and Frontier Molecular Orbital Analysis of Essential Oils from Lemongrass, Kaffir Lime, Lime, Dill, and Shatavari Against Malassezia furfur. International Journal of Molecular Sciences Vol.26 No.12 (2025). doi:10.3390/ijms26125601 Retrieved from: https://hdl.handle.net/20.500.14740/21144
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Abstract
This study explores the chemical composition and synergistic anti-fungal properties of essential oils from the aerial parts of Satavari (Asparagus racemosus Willd.), Dill (Anethum graveolens L.), and lemongrass (Cymbopogon citratus Stapf), along with the peels of Lime (Citrus aurantifolia (Christm.)) and Kaffir lime (Citrus hystrix DC), as well as the leaves of Citrus hystrix DC, against Malassezia furfur, a yeast linked to dandruff and seborrheic dermatitis. Gas chromatography-mass spectrometry (GC-MS) identified key volatile compounds within these oils. In vitro anti-fungal assays evaluated their efficacy individually and in combinations using checkerboard dilution techniques to assess synergy. Results indicated significant antifungal activity, with lemongrass exhibiting the strongest effect (MIC of 0.125% v/v). Notably, a 1:1 combination of lemongrass and kaffir lime essential oils showed synergism, reducing the MIC to 0.0625% v/v. The antifungal activity was primarily attributed to citral and citronellal, with MICs of 0.03125% v/v and 0.125% v/v, respectively. Molecular orbital analysis revealed that the higher energy levels of the lowest unoccupied molecular orbitals (LUMOs) in citral correlate with greater antifungal efficacy, likely due to its enhanced electrophilicity, facilitating nucleophilic interactions with M. furfur’s cellular components. These findings highlight potential applications of essential oil combinations in antifungal therapies.