Publication:
Antimicrobial properties of a potential probiotic lactobacillus from thai newborn feces

0
0

Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84957628214

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 98, (2015), p.S116-S122

Suggested Citation

Chimchang J., Theparee T., Ladda B., Tanasupawat S., Wongsatayanon B.T., Taweechotipatr M. Antimicrobial properties of a potential probiotic lactobacillus from thai newborn feces. Journal of the Medical Association of Thailand. Vol 98, (2015), p.S116-S122. Retrieved from: https://hdl.handle.net/20.500.14740/6071

Title

Antimicrobial properties of a potential probiotic lactobacillus from thai newborn feces

Author(s)

Chimchang J.
 Theparee T.
 Ladda B.
 Tanasupawat S.
 Wongsatayanon B.T.
 Taweechotipatr M.

Abstract

Background: Probiotics are increasingly used to treat infectious diarrhea and antibiotic-associated diarrhea. Many probiotic bacteria are classified in general such as Lactobacillus and are able to colonize the gastrointestinal tracts of infants. Objective: This study was performed to detect antimicrobial substances and activity in 200 Lactobacillus isolates obtained from healthy Thai newborn feces. Material and Method: Reuterin production was detected by the spot overlay technique and colorimetric assay. Antimicrobial activity was tested by using a well diffusion, agar method. Results: Lactobacillus strain MSMC64-1 produced reuterin and demonstrated potent antimicrobial activity against seven pathogenic indicator strains with very strong inhibitory activities against Salmonella typhi DMST 5784 and methicillin resistant Staphylococcus aureus (MRSA) DMST 20651. There was strong inhibitory activity against methicillin resistant Staphylococcus aureus (MRSA) DMST 20654, Vibrio parahaemolyticus DMST 5665 and Shigella dysenteriae DMST 15111. There was moderate to weak inhibitory activities against Vibrio cholerae DMST 2873 and Helicobacter pylori (H40). The Lactobacillus strain MSMC 64-1 showed resistance to acidic pH (pH 2, 3, 4) and tolerance to 1%, 2%, 3%, and 4% bile concentrations. Sequencing of the 16S ribosomal DNA identified the candidate’s strain as Lactobacillus reuteri with 98% sequence homology. Conclusion: The active isolate could potentially be used as a probiotic to prevent and treat enteric infections. © 2015, Medical Association of Thailand. All rights reserved.

Subject(s)

DNA 16S
 Probiotic agent
 Reuterin
 Unclassified drug
 3-hydroxypropionaldehyde
 Antiinfective agent
 Glyceraldehyde
 Probiotic agent
 Propane
 Agar diffusion
 Antibacterial activity
 Antimicrobial activity
 Article
 Bacterium identification
 Bacterium isolation
 Colorimetry
 Controlled study
 Escherichia coli
 Feces analysis
 Growth inhibition
 Helicobacter pylori
 Human
 Intestine infection
 Lactobacillus
 Lactobacillus reuteri
 Methicillin resistant Staphylococcus aureus
 Microbiological examination
 Newborn
 Nonhuman
 Polymerase chain reaction
 Salmonella enterica serovar Typhi
 Shigella dysenteriae
 Spot overlay technique
 Thailand
 Vibrio cholerae
 Vibrio parahaemolyticus
 Analogs and derivatives
 Drug effects
 Feces
 Gastrointestinal tract
 Isolation and purification
 Lactobacillus
 Male
 Microbiology
 Anti-Infective Agents
 Feces
 Gastrointestinal Tract
 Glyceraldehyde
 Humans
 Infant, Newborn
 Lactobacillus
 Male
 Methicillin-Resistant Staphylococcus aureus
 Probiotics
 Propane

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6071

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By