Publication: Antimetastatic Potential of Rhodomyrtone on Human Chondrosarcoma SW1353 Cells
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
1741427X
Other identifier(s)
2-s2.0-85089594587
Rights Holder(s)
Scopus
Bibliographic Citation
Evidence-based Complementary and Alternative Medicine. Vol 2020, (2020)
Suggested Citation
Tayeh M., Watanapokasin R. Antimetastatic Potential of Rhodomyrtone on Human Chondrosarcoma SW1353 Cells. Evidence-based Complementary and Alternative Medicine. Vol 2020, (2020). doi:10.1155/2020/8180261 Retrieved from: https://hdl.handle.net/20.500.14740/4875
Author(s)
Abstract
Chondrosarcoma is primary bone cancer, with the forceful capacity to cause local invasion and distant metastasis, and has a poor prognosis. Cancer metastasis is a complication of most cancers; it is one of the leading causes of cancer-related death. Rhodomyrtone is a pure compound that has been shown to induce apoptosis and antimetastasis in skin cancer. However, the inhibitory effect of rhodomyrtone on human chondrosarcoma cell metastasis is largely unknown. Effect of rhodomyrtone on cell viability in SW1353 cell was determined by MTT assay. Antimigration, anti-invasion, and antiadhesion were carried out to investigate the antimetastatic potential of rhodomyrtone on SW1353 cells. Gelatin zymography was performed to determine matrix metalloproteinase-2 (MMP-2) and MMP-9 activities. The effect of rhodomyrtone on the underlying mechanisms was performed by Western blot analysis. The results demonstrated that rhodomyrtone reduced cell viability of SW1353 cells at the low concentration (<3 μg/mL); cell viability was >80%. Rhodomyrtone at the subcytotoxic concentrations (0.5, 1.5, and 3 μg/mL) significantly inhibited cell migration, invasion, and adhesion of SW1353 cells in a dose-dependent fashion. Protein expression of integrin αv, integrin β3, and the downstream migratory proteins including focal adhesion kinase (FAK) and the phosphorylation of serine/threonine AKT, Ras, RhoA, Rac1, and Cdc42 were inhibited after treatment with rhodomyrtone. Moreover, we found that rhodomyrtone decreased the protein level of MMP-2 and MMP-9 as well as the enzyme activity in SW1353 cells. Meanwhile, tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 expression was increased in a dose-dependent fashion. Besides, rhodomyrtone dramatically inhibited the expression of growth factor receptor-bound protein-2 (GRB2) and the phosphorylated form of extracellular signal regulation kinase1/2 (ERK1/2) and c-Jun N-terminal kinase1/2 (JNK1/2). These results indicated that rhodomyrtone inhibited SW1353 cell migration, invasion, and metastasis by suppressing integrin αvβ3/FAK/AKT/small Rho GTPases pathway as well as downregulation of MMP-2/9 via ERK and JNK signal inhibition. These findings indicate that rhodomyrtone possessed the antimetastasis activity that may be used for antimetastasis therapy in the future. © 2020 Malatee Tayeh and Ramida Watanapokasin.
Subject(s)
Antimetastatic agent
Beta3 integrin
CD51 antigen
Focal adhesion kinase
Gelatinase A
Gelatinase B
Growth factor receptor bound protein 2
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Mitogen activated protein kinase p38
Plant medicinal product
Protein Cdc42
Protein kinase B
Rac1 protein
Ras protein
Rho guanine nucleotide binding protein
RhoA guanine nucleotide binding protein
Rhodomyrtone
Stress activated protein kinase 1
Tissue inhibitor of metalloproteinase 1
Tissue inhibitor of metalloproteinase 2
Unclassified drug
Adhesion
Aged
Article
Cell adhesion
Cell invasion
Cell migration
Cell proliferation
Cell viability
Chondrosarcoma
Down regulation
Enzyme activity
Enzyme inhibition
Female
Human
Human cell
In vitro study
Metastasis inhibition
MTT assay
Protein expression
SW1353 cell line
Transwell assay
Upregulation
Western blotting
Wound healing assay
Zymography
Beta3 integrin
CD51 antigen
Focal adhesion kinase
Gelatinase A
Gelatinase B
Growth factor receptor bound protein 2
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Mitogen activated protein kinase p38
Plant medicinal product
Protein Cdc42
Protein kinase B
Rac1 protein
Ras protein
Rho guanine nucleotide binding protein
RhoA guanine nucleotide binding protein
Rhodomyrtone
Stress activated protein kinase 1
Tissue inhibitor of metalloproteinase 1
Tissue inhibitor of metalloproteinase 2
Unclassified drug
Adhesion
Aged
Article
Cell adhesion
Cell invasion
Cell migration
Cell proliferation
Cell viability
Chondrosarcoma
Down regulation
Enzyme activity
Enzyme inhibition
Female
Human
Human cell
In vitro study
Metastasis inhibition
MTT assay
Protein expression
SW1353 cell line
Transwell assay
Upregulation
Western blotting
Wound healing assay
Zymography