Publication:
Development of Cytochrome B, a new candidate gene for a high accuracy detection of Fasciola eggs in fecal specimens

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

3044017

DOI

10.1016/j.vetpar.2019.108922

Other identifier(s)

2-s2.0-85072158994

Rights Holder(s)

Scopus

Bibliographic Citation

Veterinary Parasitology. Vol 274, (2019)

Suggested Citation

Chontananarth T., Parawat J. Development of Cytochrome B, a new candidate gene for a high accuracy detection of Fasciola eggs in fecal specimens. Veterinary Parasitology. Vol 274, (2019). doi:10.1016/j.vetpar.2019.108922 Retrieved from: https://hdl.handle.net/20.500.14740/5129

Title

Development of Cytochrome B, a new candidate gene for a high accuracy detection of Fasciola eggs in fecal specimens

Author(s)

Chontananarth T.
 Parawat J.

Abstract

Fasciolosis among domestic ruminants has resulted in a decrease in the production of milk products and has occasionally led to the deaths of young ruminants due to of acute infections. This study aimed to discriminate between the eggs of Fasciola gigantica and other trematode eggs in samples collected from ruminant feces specimens using PCR-based methods with the new candidate gene Cytochrome B (CYTB). A species-specific primer was developed with a high degree of sensitivity (3.285 pg). The primer was able to amplify the F. gigantica genomic DNA and there were no positive results with the other related trematodes (Paramphistomum sp., Orthocoelium sp., Fischoederius sp., Calicophoron sp., Echinostoma revolutum, E. cinetorchis, E. ilocanum and Isthmiophora hortensis), freshwater snails (Lymnaea auricularia, Bithynia siamensis, Indoplanorbis exustus, Melanoides tuberculata, Tarebia granifera) or definitive hosts (Bos primigenius and Bubalus bubalis). The minimum concentration of DNA from eggs that could be give a positive result was 3.285 pg. Moreover, the results of the study confirmed the existence of F. gigantica in Nakhon Pathom Province with a high prevalence (28.57%) and revealed the area of infection through epidemiological mapping. Thus, the species-specific primer and epidemiological data in this study may be helpful for use in epidemiological studies, phylogenetic studies and veterinary studies in the future. © 2019 Elsevier B.V.

Subject(s)

Genomic DNA
 Cytochrome b
 Article
 Aurochs
 Bithynia siamensis
 Bovine
 Calicophoron
 Controlled study
 Cytochrome B gene
 Echinostoma cinetorchis
 Echinostoma ilocanum
 Echinostoma revolutum
 Fasciola gigantica
 Fascioliasis
 Feces analysis
 Fischoederius
 Gene
 Gene amplification
 Indoplanorbis exustus
 Isthmiophora hortensis
 Lymnaea auricularia
 Melanoides tuberculata
 Nonhuman
 Orthocoelium
 Paramphistomum
 Phylogenetic tree
 Polymerase chain reaction
 Ruminant
 Sensitivity and specificity
 Snail
 Tarebia granifera
 Trematode
 Water buffalo
 Amino acid sequence
 Animal
 Cattle disease
 Enzymology
 Epidemiology
 Fasciola
 Fascioliasis
 Feces
 Genetics
 Isolation and purification
 Ovum
 Parasite egg count
 Parasitology
 Phylogeny
 Prevalence
 Reproducibility
 Species difference
 Thailand
 Veterinary medicine
 Amino Acid Sequence
 Animals
 Cattle
 Cattle Diseases
 Cytochromes b
 Fasciola
 Fascioliasis
 Feces
 Ovum
 Parasite Egg Count
 Phylogeny
 Prevalence
 Reproducibility of Results
 Sensitivity and Specificity
 Species Specificity
 Thailand

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URI

https://hdl.handle.net/20.500.14740/5129

Collections

Scopus: Year 1983-2021

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