Publication: The detection of tuberculosis by loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick
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Issued Date
2014
Resource Type
File Type
application/pdf
Other identifier(s)
2-s2.0-84945397281
Rights Holder(s)
Scopus
Bibliographic Citation
Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering. (2014), p.269-300
Suggested Citation
Kaewphinit T., Santiwatanakul S., Chansiri K. The detection of tuberculosis by loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick. Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering. (2014), p.269-300. doi:10.4018/978-1-4666-6363-3.ch013 Retrieved from: https://hdl.handle.net/20.500.14740/6394
Author(s)
Abstract
Tuberculosis (TB) is an airborne infectious disease caused by the bacterium Mycobacterium Tuberculosis (MTB) and is a persistent problem in developing countries. Present methods for its detection include normal or nested Polymerase Chain Reaction (PCR) followed by electrophoresis, real-time PCR, Ziehl- Neelsen staining, and culture assay. These techniques entail various disadvantages such as high cost, long assay time and use of toxic substances. Novel loop-mediated isothermal amplification (LAMP) permits DNA to be amplified rapidly under constant temperature. The combination of LAMP and chromatographic Lateral Flow Dipstick (LAMP-LFD) by using biotinylated LAMP amplicon hybridized with Fluorescein Isothiocyanate (FITC)-labeled probes are allowed to detect MTB without electrophoresis and interpreted within 3-5 min. LAMP-LFD is as highly sensitive as PCR-electrophoresis method. Based on its sensitivity, specificity, rapidity, cost effectiveness, ease of use, and convenience, LAMP-LFD could be suitable for use in early MTB detection. © 2015 by IGI Global. All rights reserved.