Publication: Cytotoxic effect and mechanism inducing cell death of α-mangostin liposomes in various human carcinoma and normal cells
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0
Issued Date
2015
Resource Type
File Type
application/pdf
ISSN
9594973
Other identifier(s)
2-s2.0-84938780546
Rights Holder(s)
Scopus
Bibliographic Citation
Anti-Cancer Drugs. Vol 26, No.8 (2015), p.824-834
Suggested Citation
Benjakul R., Kongkaneramit L., Sarisuta N., Moongkarndi P., Müller-Goymann C.C. Cytotoxic effect and mechanism inducing cell death of α-mangostin liposomes in various human carcinoma and normal cells. Anti-Cancer Drugs. Vol 26, No.8 (2015), p.824-834. doi:10.1097/CAD.0000000000000235 Retrieved from: https://hdl.handle.net/20.500.14740/6108
Abstract
The aims of this study were to develop α-mangostin liposomes as well as to evaluate their physicochemical properties and cytotoxic activity. α-Mangostin liposomes were prepared using the reverse-phase evaporation method with lipid composition of phosphatidylcholine to cholesterol at 7: 3 molar ratios; their physicochemical properties and antiproliferative activity were assessed using an MTT assay in four human carcinoma cells [that is, human lung epithelial carcinoma (Calu-3), human colon carcinoma (HT-29), human breast carcinoma (MCF-7), and human colon carcinoma (Caco-2) cells], and two human normal cells [that is, human dermal fibroblasts (HDF) and human adult low-calcium elevated temperature (HaCaT) keratinocytes]. Determinations of morphological changes and oligonucleosomal DNA fragments were also carried out. The liposomal dispersions obtained were unilamellar vesicles as confirmed by cryotransmission and freeze-fracture electron microscopy with a particle size of 114 nm and a ζ potential of -2.56 mV. The 31P-NMR spectra showed that α-mangostin molecules orientated in the phospholipid bilayer membrane. The α-mangostin could appreciably be entrapped with an efficiency and loading of 81 and 4%, respectively. The antiproliferative activity of α-mangostin liposomes in various cancer and normal cells showed a dose-dependent inhibition in all treated cell lines. The antiproliferative effect of α-mangostin liposomes was found to be associated with apoptosis, with differences in sensitivity among the cell lines treated. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
Subject(s)
Alpha mangostin liposome
Antineoplastic agent
Cholesterol
DNA fragment
Liposome
Phosphatidylcholine
Unclassified drug
Antineoplastic agent
Liposome
Mangostin
Xanthone derivative
Adult
Antiproliferative activity
Apoptosis
Article
Breast carcinoma
Carcinoma cell
Cell death
Cell structure
Colon carcinoma
Controlled study
Dispersion
Drug cytotoxicity
Drug mechanism
Electron microscopy
Fibroblast
Human
Human cell
Human tissue
Keratinocyte
Lipid composition
Lung carcinoma
MTT assay
Particle size
Phospholipid bilayer
Phosphorus nuclear magnetic resonance
Physical chemistry
Priority journal
Cell death
Cell line
Cell proliferation
Chemistry
Drug effects
Nuclear magnetic resonance spectroscopy
Tumor cell line
Antineoplastic Agents
Cell Death
Cell Line
Cell Line, Tumor
Cell Proliferation
Humans
Liposomes
Magnetic Resonance Spectroscopy
Xanthones
Antineoplastic agent
Cholesterol
DNA fragment
Liposome
Phosphatidylcholine
Unclassified drug
Antineoplastic agent
Liposome
Mangostin
Xanthone derivative
Adult
Antiproliferative activity
Apoptosis
Article
Breast carcinoma
Carcinoma cell
Cell death
Cell structure
Colon carcinoma
Controlled study
Dispersion
Drug cytotoxicity
Drug mechanism
Electron microscopy
Fibroblast
Human
Human cell
Human tissue
Keratinocyte
Lipid composition
Lung carcinoma
MTT assay
Particle size
Phospholipid bilayer
Phosphorus nuclear magnetic resonance
Physical chemistry
Priority journal
Cell death
Cell line
Cell proliferation
Chemistry
Drug effects
Nuclear magnetic resonance spectroscopy
Tumor cell line
Antineoplastic Agents
Cell Death
Cell Line
Cell Line, Tumor
Cell Proliferation
Humans
Liposomes
Magnetic Resonance Spectroscopy
Xanthones