Publication:
Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

32700

DOI

10.1021/acs.analchem.7b00255

Other identifier(s)

2-s2.0-85020652243

Rights Holder(s)

Scopus

Bibliographic Citation

Analytical Chemistry. Vol 89, No.10 (2017), p.5428-5435

Suggested Citation

Teengam P., Siangproh W., Tuantranont A., Vilaivan T., Chailapakul O., Henry C.S. Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides. Analytical Chemistry. Vol 89, No.10 (2017), p.5428-5435. doi:10.1021/acs.analchem.7b00255 Retrieved from: https://hdl.handle.net/20.500.14740/4163

Title

Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides

Author(s)

Teengam P.
 Siangproh W.
 Tuantranont A.
 Vilaivan T.
 Chailapakul O.
 Henry C.S.

Abstract

The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, a paper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregation is reported as an alternative to traditional colorimetric approaches. PNA probes are an attractive alternative to DNA and RNA probes because they are chemically and biologically stable, easily synthesized, and hybridize efficiently with the complementary DNA strands. The acpcPNA probe contains a single positive charge from the lysine at C-terminus and causes aggregation of citrate anion-stabilized silver nanoparticles (AgNPs) in the absence of complementary DNA. In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex results in dispersion of the AgNPs as a result of electrostatic repulsion, giving rise to a detectable color change. Factors affecting the sensitivity and selectivity of this assay were investigated, including ionic strength, AgNP concentration, PNA concentration, and DNA strand mismatches. The method was used for screening of synthetic Middle East respiratory syndrome coronavirus (MERS-CoV), Mycobacterium tuberculosis (MTB), and human papillomavirus (HPV) DNA based on a colorimetric paper-based analytical device developed using the aforementioned principle. The oligonucleotide targets were detected by measuring the color change of AgNPs, giving detection limits of 1.53 (MERS-CoV), 1.27 (MTB), and 1.03 nM (HPV). The acpcPNA probe exhibited high selectivity for the complementary oligonucleotides over single-base-mismatch, two-base-mismatch, and noncomplementary DNA targets. The proposed paper-based colorimetric DNA sensor has potential to be an alternative approach for simple, rapid, sensitive, and selective DNA detection. © 2017 American Chemical Society.

Subject(s)

Amino acids
 Analytic equipment
 Assays
 Color
 Colorimetry
 DNA
 Ionic strength
 Nanoparticles
 Oligonucleotides
 Paper
 Peptides
 Probes
 RNA
 Silver
 Complementary detection
 DNA and RNA detection
 Electrostatic repulsion
 Mycobacterium tuberculosis
 Nanoparticle aggregation
 Paper-based analytical devices
 Silver nanoparticles (AgNps)
 Single-base mismatch
 Nucleic acids
 Concentration
 Detection
 Nucleic Acids
 Screening
 Bacterial DNA
 DNA
 Metal nanoparticle
 Oligonucleotide
 Peptide nucleic acid
 Silver
 Virus DNA
 Chemistry
 Colorimetry
 Genetics
 Human
 Image processing
 Limit of detection
 Metabolism
 Middle East respiratory syndrome coronavirus
 Mycobacterium tuberculosis
 Nucleic acid hybridization
 Nucleotide sequence
 Paper
 Papillomaviridae
 Procedures
 Base Sequence
 Colorimetry
 DNA
 DNA, Bacterial
 DNA, Viral
 Humans
 Image Processing, Computer-Assisted
 Limit of Detection
 Metal Nanoparticles
 Middle East Respiratory Syndrome Coronavirus
 Mycobacterium tuberculosis
 Nucleic Acid Hybridization
 Oligonucleotides
 Paper
 Papillomaviridae
 Peptide Nucleic Acids
 Silver

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URI

https://hdl.handle.net/20.500.14740/4163

Collections

Scopus: Year 1983-2021

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