Publication:
Antioxidative and cardioprotective effects of Phyllanthus urinaria L. on doxorubicin-induced cardiotoxicity

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Issued Date

2005

Resource Type

Article

File Type

application/pdf

ISSN

9186158

DOI

10.1248/bpb.28.1165

Other identifier(s)

2-s2.0-24944445527

Rights Holder(s)

Scopus

Bibliographic Citation

Biological and Pharmaceutical Bulletin. Vol 28, No.7 (2005), p.1165-1171

Suggested Citation

Chularojmontri L., Wattanapitayakul S.K., Herunsalee A., Charuchongkolwongse S., Niumsakul S., Srichairat S. Antioxidative and cardioprotective effects of Phyllanthus urinaria L. on doxorubicin-induced cardiotoxicity. Biological and Pharmaceutical Bulletin. Vol 28, No.7 (2005), p.1165-1171. doi:10.1248/bpb.28.1165 Retrieved from: https://hdl.handle.net/20.500.14740/6113

Title

Antioxidative and cardioprotective effects of Phyllanthus urinaria L. on doxorubicin-induced cardiotoxicity

Author(s)

Chularojmontri L.
 Wattanapitayakul S.K.
 Herunsalee A.
 Charuchongkolwongse S.
 Niumsakul S.
 Srichairat S.

Abstract

Cardiac toxicity is a major adverse effect caused by doxorubicin (DOX) therapy. Many recent studies have shown that DOX toxicity involves generation of reactive oxygen species (ROS). Although protection or alleviation of DOX toxicity can be achieved by administration of antioxidant vitamins such as ascorbic acid and vitamin E, their cardioprotective effect remains controversial. Thus alternative naturally occurring antioxidants may potentially be candidates for antioxidant therapy. In this study, we investigated the antioxidative and cytoprotective effects of Phyllanthus urinaria (PU) against DOX toxicity using H9c2 cardiac myoblasts. The total antioxidant capacity of PU (1 mg/ml) was 5306.75±461.62 FRAP value (μM). DOX IC50 values were used to evaluate the cytoprotective effects of PU ethanolic extract (1 or 10 μg/ml) in comparison with those of ascorbic acid (VIT C, 100 μM) and N-acetylcysteine (NAC, 100 μM). PU treatments (1 or 10 μg/ml) dose dependently caused rightward DOX IC50 shifts of 2.8- and 8.5-fold, respectively while treatments with VIT C and NAC increased DOX IC50 by 3.3- and 4.2-fold, respectively. Additionally, lipid peroxidation and caspase-3 activity were parameters used to evaluate cytoprotective effect. All antioxidants completely inhibited cellular lipid peroxidation and caspase-3 activation induced by DOX (1 μM). Endogenous antioxidant defense such as total glutathione (tGSH), catalase and superoxide dismutase (SOD) activity was also modulated by the antioxidants. PU treatment alone dose dependently increased tGSH, and this effect was retained in the presence of DOX. Similar effect was observed in the assessment of catalase and SOD enzyme activity. The nuclear factor κB (NF κB) transcription factor assay demonstrated that all antioxidants significantly inhibited DOX-induced NF κB activation. Our results suggest that PU protection against DOX cardiotoxicity was mediated through multiple pathways and this plant may serve as an alternative source of antioxidants for prevention of DOX cardiotoxicity. © 2005 Pharmaceutical Society of Japan.

Subject(s)

Acetylcysteine
 Antioxidant
 Ascorbic acid
 Caspase 3
 Catalase
 Doxorubicin
 Glutathione
 Immunoglobulin enhancer binding protein
 Phyllanthus urinaria extract
 Plant extract
 Superoxide dismutase
 Transcription factor
 Unclassified drug
 Animal cell
 Antioxidant activity
 Article
 Cardiotoxicity
 Cell protection
 Controlled study
 Dose response
 Drug effect
 Enzyme activation
 Enzyme activity
 Heart protection
 IC 50
 Lipid peroxidation
 Nonhuman
 Phyllanthus
 Phyllanthus urinaria
 Rat
 Animals
 Antioxidants
 Cardiotonic Agents
 Caspase 3
 Caspases
 Cell Line
 Doxorubicin
 Heart
 Lipid Peroxidation
 NF-kappa B
 Phyllanthus
 Rats

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https://hdl.handle.net/20.500.14740/6113

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Scopus: Year 1983-2021

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