Publication:
Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

22211691

DOI

10.1016/j.apjtb.2016.01.015

Other identifier(s)

2-s2.0-84969523684

Rights Holder(s)

Scopus

Bibliographic Citation

Asian Pacific Journal of Tropical Biomedicine. Vol 6, No.6 (2016), p.539-545

Suggested Citation

Anucherngchai S., Tejangkura T., Chontananarth T. Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand. Asian Pacific Journal of Tropical Biomedicine. Vol 6, No.6 (2016), p.539-545. doi:10.1016/j.apjtb.2016.01.015 Retrieved from: https://hdl.handle.net/20.500.14740/5368

Title

Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand

Author(s)

Anucherngchai S.
 Tejangkura T.
 Chontananarth T.

Abstract

Objective: To investigate the prevalence of cercarial trematode infection in snails and to examine the reconstruction of the phylogenetic relationship to explain the molecular system of cercarial stage trematodes to estimate the infection rate of in the definite host from the Chao-Phraya Basin. Methods: The snails were collected from 10 provinces of the Chao-Phraya Basin, Thailand by stratified sampling method. The snails were examined for cercarial infection by the crushing method. All DNA specimens were amplified with internal transcribed spacer 3 (ITS3) and ITS4 primer based on PCR technique. The sequence data were aligned and used to reconstruct the phylogenetic tree by unweighted pair-group method with arithmetic means with 10. 000 bootstraps. Results: The overall rate of cercarial infection was found to be 5.90% (122/2. 067). Snails in the family Thiaridae were found to be in the highest prevalence followed by Lymnaeidae, Bithyniidae, Planorbidae, Viviparidae, and Ampullariidae, respectively, while the Buccinidae family (Clea helena) did not reveal any infections. The frequently found species of cercariae were parapleurolophocercous cercariae, cercariae and megarulous cercariae. The monophyletic tree separated the snails into five groups comprised of Heterophyidae, Strigeidae, Lecithodendriidae, Philophthalmidae and Echinostomatidae using the sequence of Angiostrongylus cantonensis as an out-group. Conclusions: This study was the first to report on cercarial infection in the Chao-Phraya Basin, Thailand. This revealed that a high variety of freshwater snails were infected by cercariae stage trematodes with a high prevalence. The sequence data of ITS2 can be used to investigate the phylogenetic relationships of trematodes at the family level and in each clade of different families separated by the definitive hosts. © 2016 Hainan Medical University.

Subject(s)

Genomic DNA
 Internal transcribed spacer
 Internal transcribed spacer 3
 Internal transcribed spacer 4
 Unclassified drug
 Ampullariidae
 Angiostrongylus cantonensis
 Article
 Bithyniidae
 Buccinidae
 Cercaria
 Controlled study
 Echinostomatidae
 Echinostome cercaria
 Freshwater species
 Furcocercous cercaria
 Gene amplification
 Genome size
 Heterophyidae
 Host pathogen interaction
 Infection rate
 Lecithodendriidae
 Lymnaeidae
 Megarulous cercaria
 Molecular diagnosis
 Molecular phylogeny
 Monophyly
 Monostome cercaria
 Nonhuman
 Nucleotide sequence
 Parapleurolophocercous cercaria
 Philophthalmidae
 Phylogenetic tree
 Planorbidae
 Pleurolophocercous cercaria
 Polymerase chain reaction
 Prevalence
 Priority journal
 Sequence alignment
 Snail
 Stratified sample
 Strigeidae
 Thailand
 Trematode
 Trematode life cycle stage
 Trematodiasis
 Virgulate cercaria
 Viviparidae
 Xiphidiocercaria cercaria

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URI

https://hdl.handle.net/20.500.14740/5368

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Scopus: Year 1983-2021

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