Publication:
Anti-proliferation and apoptosis induction in epidermoid carcinoma A431 cells by Artonin E

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-85075028775

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S54-S60

Suggested Citation

Innajak S., Tangchirakhaphan S., Nilwarangoon S., Tanjapatkul N., Mahabusarakam W., Watanapokasin R. Anti-proliferation and apoptosis induction in epidermoid carcinoma A431 cells by Artonin E. Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S54-S60. Retrieved from: https://hdl.handle.net/20.500.14740/4314

Title

Anti-proliferation and apoptosis induction in epidermoid carcinoma A431 cells by Artonin E

Author(s)

Innajak S.
 Tangchirakhaphan S.
 Nilwarangoon S.
 Tanjapatkul N.
 Mahabusarakam W.
 Watanapokasin R.

Abstract

Background: Skin cancer is the type of cancer that is becoming an increasingly important public health problem worldwide. The treatment for skin cancer depends on the stage and location of cancer cells. Therefore, the new finding of anti-cancer compound as a therapeutic candidate for skin cancer is necessary. Objective: To investigate the effect of artonin E on anti-proliferation and apoptosis induction in skin cancer epidermoid carcinoma A431 cells. Material and Method: Cell viability and cell proliferation were determined by MTT assay. Nuclear morphological changes, mitochondrial membrane potential and protein expression were determined by Hoechst 33342 and JC-1 staining, respectively. Protein expression was determined by Western blot analysis. Results: Artonin E showed anti-proliferation in A431 treated cells in a dose-dependent manner with an IC50 value of 9.05+6.15 μg/ml. In addition, artonin E induced chromatin condensation and apoptotic bodies in A431 treated cells. JC-1 staining showed that artonin E induced loss of mitochondrial membrane potential. Western blot analysis showed the upregulation of cleaved-caspaase-7 and cleaved-PARP in A431 treated cells. Conclusion: In this study, artonin E showed anti-proliferation and apoptosis induction in A431 treated cells. Moreover, artonin E induced cleaved caspase-7 and cleaved-PARP activation in A431 cells, resulting in apoptosis cell death. Our results indicated that artonin E may be further developed as an anti-cancer drug and the underlying mechanisms of apoptosis induction in A431 cells should be studied. © 2017 Medical Association of Thailand. All rights reserved.

Subject(s)

Artonin e
 Caspase 7
 Flavonoid
 Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
 Unclassified drug
 A-431 cell line
 Anti proliferation activity
 Antineoplastic activity
 Apoptosis
 Article
 Cell proliferation
 Cell proliferation assay
 Cell structure
 Cell viability
 Cell viability assay
 Chromatin condensation
 Controlled study
 Cytotoxicity
 Drug activity
 Fluorescence microscopy
 Human
 Human cell
 IC50
 Mitochondrial membrane potential
 MTT assay
 Proapoptotic activity
 Protein expression
 Skin cancer
 Squamous cell carcinoma
 Upregulation
 Western blotting

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URI

https://hdl.handle.net/20.500.14740/4314

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Scopus: Year 1983-2021

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