Publication: Isolation of bacteriophages specific to Pseudomonas mosselii for controlling milk spoilage
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Issued Date
2023
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application/pdf
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Scopus
Bibliographic Citation
International Dairy Journal. Vol 145, No. (2023)
Suggested Citation
Wongyoo R., Sunthornthummas S., Sawaengwong T., Surachat K., Rangsiruji A., Atithep T., Sarawaneeyaruk S., Doi K., Nantavisai K., Insian K., Pomwised R., Pringsulaka O. Isolation of bacteriophages specific to Pseudomonas mosselii for controlling milk spoilage. International Dairy Journal. Vol 145, No. (2023). doi:10.1016/j.idairyj.2023.105674 Retrieved from: https://hdl.handle.net/20.500.14740/12351
Other Contributor(s)
Abstract
Bacteriophages controlling heat-stable protease producing Pseudomonas mosselii were characterised and applied. Two Podoviridae family phages, designated ΦC106 and Φ21A, were isolated and shown to exhibit a broad host range. The optimal multiplicity of infection of both phages generating maximum titres was 10, latent periods were at 20 min and burst sizes were 1862 and 331 plaque-forming units per infected cell for ΦC106 and Φ21A, respectively. ΦC106 and Φ21A genomes did not encode known toxins, antibiotic-resistant genes, virulent factors of bacterial origin, or lysogenic markers such as integrase, recombinase, repressor/anti-repressor protein, and excisionase, guaranteeing their safety for addition into milk and dairy products. In vitro lytic activity and proteolytic activity of ΦC106 and Φ21A revealed a remarkable decrease in bacterial count and enzyme activity. These data suggest that ΦC106 and Φ21A are efficient in controlling the prevalence of spoilage-causing P. mosselii strains in dairy- and food-related environments. © 2023 Elsevier Ltd