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Differentiation among the Vibrio cholerae serotypes O1, O139, O141 and non-O1, non-O139, non-O141 using specific monoclonal antibodies with dot blotting

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

1677012

DOI

10.1016/j.mimet.2011.07.022

Other identifier(s)

2-s2.0-80053573745

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Microbiological Methods. Vol 87, No.2 (2011), p.224-233

Suggested Citation

Pengsuk C., Longyant S., Rukpratanporn S., Chaivisuthangkura P., Sridulyakul P., Sithigorngul P. Differentiation among the Vibrio cholerae serotypes O1, O139, O141 and non-O1, non-O139, non-O141 using specific monoclonal antibodies with dot blotting. Journal of Microbiological Methods. Vol 87, No.2 (2011), p.224-233. doi:10.1016/j.mimet.2011.07.022 Retrieved from: https://hdl.handle.net/20.500.14740/7224

Title

Differentiation among the Vibrio cholerae serotypes O1, O139, O141 and non-O1, non-O139, non-O141 using specific monoclonal antibodies with dot blotting

Author(s)

Pengsuk C.
 Longyant S.
 Rukpratanporn S.
 Chaivisuthangkura P.
 Sridulyakul P.
 Sithigorngul P.

Abstract

Seven different monoclonal antibodies (MAbs) specific to only Vibrio cholerae were produced using a combination of five representative serotypes of V. cholerae for immunization. The first three MAbs (VC-93, VC-82 and VC-223) were specific to the V. cholerae serogroup O1 with different avidity for the serotypes O1 Inaba and O1 Ogawa. The fourth and the fifth MAbs were specific to V. cholerae O139 (VC-812) or O141 (VC-191) serogroups, respectively. The sixth MAb (VC-26) bound to all three serogroups of V. cholerae. The seventh MAb (VC-63) bound to all twenty five isolates of V. cholerae used in this study. None of the seven MAbs showed cross-reactivity with other Vibrio spp. or closely-related V. cholerae species, V. mimicus or other gram-negative bacteria. The eighth MAbs (VC-201) specific to almost all Vibrio spp. was also obtained. In dot blotting, these MAbs can be used to detect a diluted pure culture of V. cholerae in solution with a sensitivity range of from 10 5 to 10 7CFUml -1. However, the detection capability could be improved equivalent to that of PCR technique after preincubation of samples in alkaline peptone water (APW). Thus, these MAbs constitute convenient immunological tools that can be used for simple, rapid and simultaneous direct detection and differentiation of the individual serotypes of V. cholerae in complex samples, such as food and infected animals, without the requirement for bacterial isolation or biochemical characterization. © 2011 Elsevier B.V.

Subject(s)

Monoclonal antibody
 Peptone
 Article
 Bacterium culture
 Bacterium isolate
 Bacterium isolation
 Blotting
 Colony forming unit
 Controlled study
 Cross reaction
 Dot blotting
 Gram negative bacterium
 Immunization
 Nonhuman
 Polymerase chain reaction
 Priority journal
 Serotype
 Vibrio cholerae
 Vibrio mimicus
 Animals
 Antibodies, Bacterial
 Antibodies, Monoclonal
 Antigens, Bacterial
 Cholera
 Feces
 Female
 Food Microbiology
 Humans
 Immunoblotting
 Mice
 Palaemonidae
 Serotyping
 Vibrio cholerae
 Water Microbiology
 Animalia
 Bacteria (microorganisms)
 Negibacteria
 Vibrio
 Vibrio cholerae
 Vibrio mimicus

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URI

https://hdl.handle.net/20.500.14740/7224

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Scopus: Year 1983-2021

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