Publication: Exosome aggregation mediated stop-flow paper-based portable device for rapid exosome quantification
0
0
Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
1730835
Other identifier(s)
2-s2.0-85078672314
Rights Holder(s)
Scopus
Bibliographic Citation
Electrophoresis. Vol 41, (2020), p.311-318
Suggested Citation
Chutvirasakul B., Nuchtavorn N., Suntornsuk L., Zeng Y. Exosome aggregation mediated stop-flow paper-based portable device for rapid exosome quantification. Electrophoresis. Vol 41, (2020), p.311-318. doi:10.1002/elps.201900323 Retrieved from: https://hdl.handle.net/20.500.14740/4647
Author(s)
Abstract
Exosome quantification is important for estimation of informative messengers (e.g., proteins, lipids, RNA, etc.) involving physiological and pathological effects. This work aimed to develop a simple and rapid distance-based paper portable device using exosome-capture vesicles (polydiacetylene conjugated with antiCD81) for exosome quantification in cell cultures. This novel concept relied on distinct aggregation of exosomes and exosome-capture vesicles leading to different solvent migration. Distances of the migration were used as signal readouts, which could be detected by naked eye. PDA-antiCD81 as exosome-capture vesicles were optimized (e.g., size, reaction ratio, and concentration) and the paper designs were investigated (e.g., diameter of sample reservoir and lamination layer) to enhance the solvent stop-flow effects. Finally, exosome screening on three cell culture samples (COLO1, MDA-MB-231, and HuR-KO1 subclone) was demonstrated. The method could linearly measure exosome concentrations in correlation with solvent migration distances in the range of 106–1010 particles/mL (R2 > 0.98) from the cell culture samples. The exosome concentration measurements by the developed device were independently assessed by nanoparticle tracking analysis. Results demonstrated no statistically significant difference (p > 0.05) by t-test. This low-cost and rapid device allows a portable platform for exosome quantification without the requirement of expensive equipment and expertise of operation. The developed device could potentially be useful for quantification of other biomarker-related extracellular vesicles. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Subject(s)
CD81 antigen
Polyacetylene derivative
Article
Cell aggregation
Cell assay
Cell clone
Cell culture
Cell vacuole
COLO1 cell line
Concentration (parameter)
Controlled study
Equipment design
Exosome
Exosome capture vesicle
HuR-KO1 cell line
MDA-MB-231 cell line
Process development
Process optimization
Stop flow paper based portable device
Polyacetylene derivative
Article
Cell aggregation
Cell assay
Cell clone
Cell culture
Cell vacuole
COLO1 cell line
Concentration (parameter)
Controlled study
Equipment design
Exosome
Exosome capture vesicle
HuR-KO1 cell line
MDA-MB-231 cell line
Process development
Process optimization
Stop flow paper based portable device