Publication:
Activation of chloride secretion by isoflavone genistein in endometrial epithelial cells

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

10158987

DOI

10.1159/000356584

Other identifier(s)

2-s2.0-84890688990

Rights Holder(s)

Scopus

Bibliographic Citation

Cellular Physiology and Biochemistry. Vol 32, No.5 (2013), p.1473-1486

Suggested Citation

Deachapunya C., Poonyachoti S. Activation of chloride secretion by isoflavone genistein in endometrial epithelial cells. Cellular Physiology and Biochemistry. Vol 32, No.5 (2013), p.1473-1486. doi:10.1159/000356584 Retrieved from: https://hdl.handle.net/20.500.14740/6844

Title

Activation of chloride secretion by isoflavone genistein in endometrial epithelial cells

Author(s)

Deachapunya C.
 Poonyachoti S.

Abstract

Background/Aim: Genistein, the most active isoflavone found primarily in soybeans, alters ion transport functions in intestinal and airway epithelia. The present study aims to investigate the acute effects and mechanisms of action of genistein in immortalized porcine endometrial epithelial cells. Methods: Ussing chamber technique was used for transepithelial electrical measurements. Results: Genistein increased short-circuit currents (I sc ) which were inhibited by glibenclamide, NPPB, CFTRinh-172, DIDS or bumetanide, but not amiloride. In experiments with amphotericin B-permeabilized monolayers, genistein activated the apical Cl - current and barium-sensitive basolateral K + current while inhibiting the apical K + current. Genistein failed to increase the I sc in the presence of forskolin or IBMX, but did increase the I sc in UTP. Pretreatment with genistein also abolished the increase in the I sc when induced by forskolin, IBMX or UTP. However, Ca 2+ -chelating BAPTA-AM did not affect the genistein-induced increase in the I sc . The genistein-stimulated I sc was reduced by tyrosine kinase inhibitors, tyrphostin A23 or AG490. However, vanadate, a tyrosine phosphatase inhibitor, failed to inhibit the genistein response. Estrogen receptor antagonist ICI182,780 did not alter the genistein's action. Conclusion: The soy isoflavone, genistein, stimulates Cl - secretion in endometrial epithelial cells possibly via a direct activation of CFTR which appears to be modulated through a tyrosine kinase-dependent pathway. The present findings may be of benefit for the therapeutic application of genistein in the treatment of electrolyte transport disorders in the epithelia. © 2013 S. Karger AG, Basel.

Subject(s)

1,2 bis(o aminophenoxy)ethane n,n,n',n' tetraacetic acid
 4,4' diisothiocyanatostilbene 2,2' disulfonic acid
 5 nitro 2 (3 phenylpropylamino)benzoic acid
 Acetic acid derivative
 Amiloride
 Amphotericin B
 Antiestrogen
 Barium
 Bumetanide
 Chloride
 Daidzein
 Forskolin
 Genistein
 Glibenclamide
 Isobutylmethylxanthine
 Tyrphostin
 Unclassified drug
 Uridine triphosphate
 Vanadic acid
 4,4' diisothiocyanatostilbene 2,2' disulfonic acid
 Bumetanide
 Cystic fibrosis transmembrane conductance regulator
 Ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid
 Forskolin
 Fulvestrant
 Glibenclamide
 Isobutylmethylxanthine
 Isoflavone
 N benzyl 2 cyano 3 (3,4 dihydroxyphenyl)acrylamide
 Protein tyrosine kinase
 Tyrphostin
 Uridine triphosphate
 Vanadic acid
 Animal cell
 Animal cell culture
 Apical membrane
 Article
 Basolateral membrane
 Chloride transport
 Controlled study
 Endometrium cell
 Membrane permeability
 Nonhuman
 Phosphorylation and dephosphorylation
 Potassium current
 Potential difference
 Priority journal
 Short circuit current
 Article
 Cell immortalization
 Chloride current
 Drug mechanism
 Endometrium
 Female
 Monolayer culture
 Swine
 Glycine max
 Sus
 1-Methyl-3-isobutylxanthine
 Amiloride
 Animals
 Bumetanide
 Cell Membrane Permeability
 Cells, Cultured
 Chlorides
 Colforsin
 Cystic Fibrosis Transmembrane Conductance Regulator
 Egtazic Acid
 Endometrium
 Epithelial Cells
 Estradiol
 Female
 Genistein
 Glyburide
 Swine
 Uridine Triphosphate

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URI

https://hdl.handle.net/20.500.14740/6844

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Scopus: Year 1983-2021

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