Publication:
Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences

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Issued Date

1999

Resource Type

Article

File Type

application/pdf

ISSN

3044017

DOI

10.1016/S0304-4017(99)00052-7

Other identifier(s)

2-s2.0-0033036561

Rights Holder(s)

Scopus

Bibliographic Citation

Veterinary Parasitology. Vol 83, No.2 (1999), p.99-105

Suggested Citation

Chansiri K., Kawazu S.-I., Kamio T., Terada Y., Fujisaki K., Philippe H., Sarataphan N. Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences. Veterinary Parasitology. Vol 83, No.2 (1999), p.99-105. doi:10.1016/S0304-4017(99)00052-7 Retrieved from: https://hdl.handle.net/20.500.14740/7136

Title

Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences

Author(s)

Chansiri K.
 Kawazu S.-I.
 Kamio T.
 Terada Y.
 Fujisaki K.
 Philippe H.
 Sarataphan N.

Abstract

Classification of Theileria parasites of south-east Asian countries is still ambiguous due to the lack of basic studies, especially their molecular genetic information. In this study, we included 6 known species and 14 unclassified Theileria parasite isolates: Theileria annulata, Theileria parva, Theileria taurotragi, Theileria sergenti, Theileria buffeli, Theileria types Sable, Theileria types A, B, B1, B2, C, D, E, F, G, G1, Theileria type Medan (Indonesia), Theileria type Ipoh (Malaysia) and Theileria type Thong Song (Thailand). Small subunit ribosomal RNA (srRNA) nucleotide sequence data were collected by PCR, cloning and dideoxy sequencing. The srRNA nucleotide sequences were aligned and analyzed by distance methods, maximum parsimony algorithms and maximum likelihood methods to construct phylogenetic trees. Bootstrap analysis was used to test the strength of the different phylogenetic reconstructions. The data indicated that all of the tree-building methods gave very similar results. This study identified two groups of Theileria, the pathogenic and benign groups, which are strongly supported by bootstrap analysis. The analysis also indicated that three subgroups (A, B and C) were generated within the benign Theileria group whereas the classification of Theileria type D and Thong Song is questionable. However, more basic information such as life cycle differences, vectors, modes of transmission, virulent and genetic/sexual compatability is essential for clearer taxonomic definition of the benign Theileria parasites. Copyright (C) 1999 Elsevier Science B.V.

Subject(s)

Ribosome DNA
 Animal experiment
 Article
 Nonhuman
 Nucleotide sequence
 Parasite identification
 Phylogeny
 Polymerase chain reaction
 Theileria
 Theileria parva
 Animals
 Asia, Southeastern
 Base Sequence
 DNA, Protozoan
 Molecular Sequence Data
 Phylogeny
 Polymerase Chain Reaction
 RNA, Ribosomal
 Sequence Alignment
 Sequence Analysis, DNA
 Sequence Homology, Nucleic Acid
 Theileria
 Theileriasis
 Martes zibellina
 Theileria
 Theileria annulata
 Theileria buffeli
 Theileria parva
 Theileria sergenti
 Theileria taurotragi
 Unclassified Theileria

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URI

https://hdl.handle.net/20.500.14740/7136

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Scopus: Year 1983-2021

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