Publication:
Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers

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Issued Date

1998

Resource Type

Article

File Type

application/pdf

ISSN

3044017

DOI

10.1016/S0304-4017(98)00155-1

Other identifier(s)

2-s2.0-0031691332

Rights Holder(s)

Scopus

Bibliographic Citation

Veterinary Parasitology. Vol 79, No.2 (1998), p.143-149

Suggested Citation

Chansiri K., Kawazu S.-I., Kamio T., Fujisaki K., Panchadcharam C., Watanapokasin Y., Uthaisang W., Tananyutthawongese C., Sarataphan N. Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers. Veterinary Parasitology. Vol 79, No.2 (1998), p.143-149. doi:10.1016/S0304-4017(98)00155-1 Retrieved from: https://hdl.handle.net/20.500.14740/7190

Title

Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers

Author(s)

Chansiri K.
 Kawazu S.-I.
 Kamio T.
 Fujisaki K.
 Panchadcharam C.
 Watanapokasin Y.
 Uthaisang W.
 Tananyutthawongese C.
 Sarataphan N.

Abstract

A method was developed to obtain reproducible DNA fingerprints from five distinct purified benign Theileria genomic DNAs by PCR-based amplification. Randomly amplified polymorphic DNA (RAPD) profiles were obtained from 10 randomly designed 12-mers. However, nine of the 10 primers could generate the difference in RAPD-PCR profiles which allowed discrimination of Theileria species. The method has advantage of being simple, fast and sensitive for diagnosis and characterization of the parasites since it does not require prior DNA sequence information to construct species-specific probes or primers. The results are also beneficial for a proper understanding of the epidemiology and designing rational control programmes for Theileriosis in Asian and South-East Asian countries. Copyright (C) 1998 Elsevier Science B.V.

Subject(s)

Animal parasitosis
 Article
 Cattle
 Diagnostic procedure
 Dna fingerprinting
 Genome
 Nonhuman
 Polymerase chain reaction
 Random amplified polymorphic dna
 Species differentiation
 Theileria
 Animals
 Asia, Southeastern
 Australia
 Cattle
 DNA Fingerprinting
 DNA Primers
 DNA, Protozoan
 Electrophoresis, Agar Gel
 Genome, Protozoan
 Japan
 Random Amplified Polymorphic DNA Technique
 Reproducibility of Results
 Species Specificity
 Theileria
 Theileriasis
 Theileria

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URI

https://hdl.handle.net/20.500.14740/7190

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Scopus: Year 1983-2021

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