Publication: Protective effects of ethyl acetate extract of Eclipta prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells
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Issued Date
2016
Resource Type
File Type
application/pdf
ISSN
15131874
Other identifier(s)
2-s2.0-85006841267
Rights Holder(s)
Scopus
Bibliographic Citation
ScienceAsia. Vol 42, No.4 (2016), p.259-265
Suggested Citation
Jaisin Y., Ratanachamnong P., Prachayasittikul S., Watanapokasin R., Kuanpradit C. Protective effects of ethyl acetate extract of Eclipta prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells. ScienceAsia. Vol 42, No.4 (2016), p.259-265. doi:10.2306/scienceasia1513-1874.2016.42.259 Retrieved from: https://hdl.handle.net/20.500.14740/5270
Abstract
Oxidative stress plays an important role in the pathogenesis of Parkinson's disease. The neurotoxin 6-hydroxydopamine initiates cellular oxidative stress which has been implicated in neurodegenerative conditions related to Parkinson's disease. Eclipta prostrata, also known as false daisy, is a plant belonging to the sunflower family. It is a rich source of phenolic compounds that confer the plant an antioxidant activity. In this study, we investigated the protective effects of ethyl acetate extract of E. prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells. Pretreatment with the ethyl acetate extract of E. prostrata before exposing the cells to 6-hydroxydopamine significantly increased cell survival and reduced intracellular reactive oxygen species formation in a concentration-dependent manner. In addition, the ethyl acetate extract of E. prostrata suppressed the Bax/Bcl-2 ratio and reduced caspase-3 activity, a key modulator of cell apoptosis. Our finding clearly shows that the ethyl acetate extract of E. prostrata possesses a potential antioxidant capacity and attenuates SH-SY5Y cell death by blocking apoptotic signalling caused by 6-hydroxydopamine. E. prostrata could therefore be used as a potential herb to prevent oxidative stress-induced neurodegeneration in Parkinson's disease. © 2016, Science Society of Thailand under Royal Patronage. All rights reserved.