Publication:
Echinostoma revolutum: Development of a high performance DNA-specific primer to demonstrate the epidemiological situations of their intermediate hosts

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

0001706X

DOI

10.1016/j.actatropica.2018.09.014

Other identifier(s)

2-s2.0-85054439028

Rights Holder(s)

Scopus

Bibliographic Citation

Acta Tropica. Vol 189, (2019), p.46-53

Suggested Citation

Anucherngchai S., Chontananarth T. Echinostoma revolutum: Development of a high performance DNA-specific primer to demonstrate the epidemiological situations of their intermediate hosts. Acta Tropica. Vol 189, (2019), p.46-53. doi:10.1016/j.actatropica.2018.09.014 Retrieved from: https://hdl.handle.net/20.500.14740/5575

Title

Echinostoma revolutum: Development of a high performance DNA-specific primer to demonstrate the epidemiological situations of their intermediate hosts

Author(s)

Anucherngchai S.
 Chontananarth T.

Abstract

Echinostomiasis caused by the Echinostoma group, in particular E. revolutum are a significant problem for both humans and other animals. This group has a large number of morphological similarities that are difficult and time-consuming to identify. The present study aimed to develop high-performance tools for the detection of the prevalence of E. revolutum and to reveal the prevalence of E. revolutum infections in intermediate snail hosts in Lopburi province, Thailand. The snail specimens were collected by stratified sampling method and examined to collect trematodes in the larval stage. The specific primer was manually designed and based on 18 s rDNA and verified the specificity and sensitivity for use as an identification tool to compare with classical method, constructed by epidemic mapping. The overall prevalence value of E. revolutum was found to be 16.26%. Tha Luang district had the highest prevalence (70.14%), followed by Chai Badan, Phatthana Nikhom, Tha Wung, Ban Mi, Khok Samrong, Nong Muang and Sa Bot at 42%, 25.14%, 2.52%, 1.73%, 2%, 1.33% and 0.40%, respectively. With regard to the specific primer, it can amplify both cercarial and metacercarial DNA (90 pg/μl.) and discriminated E. revolutum from its hosts, other trematodes and other echinostome larvae with no cross-reactions. Therefore, the developed specific primer can be used as a species-specific identification tool with a high degree of sensitivity and specificity. Consequently, this data is important for monitoring the outbreak of E. revolutum. It can be applied for initiating surveillance programs of snail-borne diseases in both medical and veterinary studies. © 2018 Elsevier B.V.

Subject(s)

DNA
 DNA 18S
 Primer DNA
 Primer DNA
 Biotechnology
 Detection method
 Epidemiology
 Intermediate host
 Parasite prevalence
 Snail
 Animal experiment
 Article
 Cercaria
 Controlled study
 Cross reaction
 Demography
 Echinostoma revolutum
 Echinostomatidae
 Echinostomiasis
 Intermediate host
 Larval stage
 Metacercaria
 Nonhuman
 Polymerase chain reaction
 Prevalence
 Sampling
 Sensitivity and specificity
 Snail
 Thailand
 Trematode
 Animal
 Echinostoma
 Echinostomiasis
 Epidemic
 Genetics
 Isolation and purification
 Parasitology
 Species difference
 Veterinary medicine
 Central Region [Thailand]
 Lopburi
 Thailand
 Animalia
 Echinostoma
 Echinostoma revolutum
 Gastropoda
 Trematoda
 Animals
 Cercaria
 Disease Outbreaks
 DNA Primers
 Echinostoma
 Echinostomiasis
 Metacercariae
 Prevalence
 Snails
 Species Specificity
 Thailand

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URI

https://hdl.handle.net/20.500.14740/5575

Collections

Scopus: Year 1983-2021

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