Publication:
Biodegradable nanoparticles surface modification techniques with cIBR peptide targeting to LFA-1 expressing leukemic cells

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

19492944

DOI

10.1115/1.4023896

Other identifier(s)

2-s2.0-84881137715

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Nanotechnology in Engineering and Medicine. Vol 3, No.4 (2012), p.-

Suggested Citation

Phongpradist R., Chittasupho C., Intasai N., Siahaan T.J., Berkland C.J., Charoenkwan P., Anuchapreeda S., Ampasavate C. Biodegradable nanoparticles surface modification techniques with cIBR peptide targeting to LFA-1 expressing leukemic cells. Journal of Nanotechnology in Engineering and Medicine. Vol 3, No.4 (2012), p.-. doi:10.1115/1.4023896 Retrieved from: https://hdl.handle.net/20.500.14740/6930

Title

Biodegradable nanoparticles surface modification techniques with cIBR peptide targeting to LFA-1 expressing leukemic cells

Author(s)

Phongpradist R.
 Chittasupho C.
 Intasai N.
 Siahaan T.J.
 Berkland C.J.
 Charoenkwan P.
 Anuchapreeda S.
 Ampasavate C.

Abstract

The lymphocyte function associated antigen-1 (LFA-1) is evaluated for a targeting carrier in leukemia. The cIBR peptide was utilized as the targeting moiety for the drug carrier in direct targeting to LFA-1 expressing cancer cells. This study aims to evaluate the effects of the cIBR peptide conjugation on the specific targeting delivery to the leukemic cell line. Poly (D, L lactide-co-glycolide) (PLGA) nanoparticles were conjugated to the cIBR peptide by three different approaches (coupling, head, and tail) in order to evaluate the nanoparticles' characters, targetability, uptake, drug releasing, and cytotoxicity of each approach. The prepared PLGA nanoparticles were spherical lin shape with a size range of 200-450 nm. The targetability and uptake of three types of cIBR-conjugated nanoparticles (cIBR-NPs) were evidenced and quantified by flow cytometry. The coupling approach presented the highest targetability, uptake, drug releasing, and cytotoxicity followed by the head and tail approaches, respectively. The peptide conjugation method onto the nanoparticles surface was proven to be a key factor for the nanoparticles' physicochemical characteristicss and their efficient delivery. Copyright © 2013 by ASME.

Subject(s)

Biodegradable nanoparticle
 Conjugation method
 Drug carrier
 Leukemia
 Paclitaxel
 PLGA nanoparticles
 Surface modification techniques
 Targeting deliveries
 Antigens
 Cell culture
 Copolymers
 Diseases
 Function evaluation
 Lymphocytes
 Peptides
 Nanoparticles
 Carboxyl group
 CIBR peptide
 CIBR peptide polyglactin nanoparticle conjugate
 Cyclopeptide
 Drug carrier
 Lymphocyte function associated antigen 1
 Nanoparticle
 Paclitaxel
 Polyglactin nanoparticle
 Unclassified drug
 Article
 Biodegradability
 Cell viability
 Chemical modification
 Chemical procedures
 Controlled study
 Coupling conjugation
 Drug binding
 Drug conjugation
 Drug cytotoxicity
 Drug delivery system
 Drug dosage form comparison
 Drug effect
 Drug formulation
 Drug release
 Drug safety
 Drug specificity
 Drug stability
 Drug targeting
 Drug uptake
 Emulsion
 Evaporation
 Flow cytometry
 Head conjugation
 IC 50
 In vitro study
 Internalization
 Leukemia cell
 Nanoencapsulation
 Particle size
 Physical chemistry
 Solvent displacement
 Surface charge
 Surface property
 Tail conjugation
 Zeta potential

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URI

https://hdl.handle.net/20.500.14740/6930

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Scopus: Year 1983-2021

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