Publication: Anti-proliferation and apoptosis induction in epidermoid carcinomaA431Cellsbyterminaliabelliricaextract
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Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85074659549
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 102, No.7 (2019)
Suggested Citation
Innajak S., Chulasiri M., Watanapokasin R. Anti-proliferation and apoptosis induction in epidermoid carcinomaA431Cellsbyterminaliabelliricaextract. Journal of the Medical Association of Thailand. Vol 102, No.7 (2019). Retrieved from: https://hdl.handle.net/20.500.14740/5525
Author(s)
Abstract
Background: Squamous cell carcinoma (SCC) is a member of non-melanoma skin cancer (NMSC), which is slow-growing and high metastasis. UV exposed from the sun or tanning beds is one of the risk factors of SCC developing. Nowadays, SCC treatments include surgery, photodynamic therapy, radiation therapy and chemotherapy, but it is unsatisfactory. Therefore the finding of new therapeutic target is necessary. Objective: To investigate the effect of Terminalia bellirica extract on anti-proliferation and apoptosis induction in highly metastasis epidermoid carcinoma A431 cells. Materials and Methods: Cytotoxicity, cell viability and cell proliferation were determined by MTT assay. Nuclear morphological changes and protein expression were determined by Hoechst staining and Western blot analysis, respectively. Results: T. bellirica extract showed anti-proliferative effect on A431 cells. Hoechst staining revealed chromatin condensation and apoptotic bodies induction in A431 treated cells by T. bellirica extract. In addition, apoptosis induction via up-regulartion of Bax, Bik, and Bok as well as down-regulation of Mcl-1 was obtained. Moreover, T. bellirica extract showed apoptosis induction through caspase-7 protein activation. Conclusion: The present study showed that T. bellirica extract inhibited cell proliferation and induced apoptosis induction in A431 treated cells. The results indicated that T. bellirica extract may be further developed as skin cancer protection product. However the underlying mechanisms of apoptosis induction in A431 cells should be further studied. © JOURNAL OF THE MEDICAL ASSOCIATION OF Thailand| 2019
Subject(s)
Antineoplastic agent
Bcl2 interacting killer protein
Bcl2 related ovarian killer protein
Caspase 7
Peptides and proteins
Plant extract
Protein Bax
Protein mcl 1
Terminalia bellirica extract
Unclassified drug
A-431 cell line
Antiproliferative activity
Apoptosis
Article
Cell proliferation
Cell structure
Cell viability
Chromatin condensation
Controlled study
Cytotoxicity
Down regulation
Enzyme activation
Fluorescence microscopy
Human
Human cell
IC50
MTT assay
Protein expression
Squamous cell carcinoma
Terminalia bellirica
Upregulation
Western blotting
Bcl2 interacting killer protein
Bcl2 related ovarian killer protein
Caspase 7
Peptides and proteins
Plant extract
Protein Bax
Protein mcl 1
Terminalia bellirica extract
Unclassified drug
A-431 cell line
Antiproliferative activity
Apoptosis
Article
Cell proliferation
Cell structure
Cell viability
Chromatin condensation
Controlled study
Cytotoxicity
Down regulation
Enzyme activation
Fluorescence microscopy
Human
Human cell
IC50
MTT assay
Protein expression
Squamous cell carcinoma
Terminalia bellirica
Upregulation
Western blotting