Publication:
PCR based method for identification of zoonostic Brugia malayi microfilariae in domestic cats

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

8908508

DOI

10.1006/mcpr.2001.0402

Other identifier(s)

2-s2.0-0036018473

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Molecular and Cellular Probes. Vol 16, No.2 (2002), p.129-135

Suggested Citation

Chansiri K., Tejangkura T., Kwaosak P., Sarataphan N., Phantana S., Sukhumsirichart W. PCR based method for identification of zoonostic Brugia malayi microfilariae in domestic cats. Molecular and Cellular Probes. Vol 16, No.2 (2002), p.129-135. doi:10.1006/mcpr.2001.0402 Retrieved from: https://hdl.handle.net/20.500.14740/6787

Title

PCR based method for identification of zoonostic Brugia malayi microfilariae in domestic cats

Author(s)

Chansiri K.
 Tejangkura T.
 Kwaosak P.
 Sarataphan N.
 Phantana S.
 Sukhumsirichart W.

Abstract

The survey of 326 human blood samples in the endemic area of Surat Thani and Narathiwat, the provinces in the south of Thailand, revealed that 5 of them were infected with Brugia malayi. Similarly, 53 feline blood samples were also investigated and found that 15 of the domestic cats were also infected with B. malayi. Upon the examination of human and feline blood specimens, a pair of human and domestic cat stayed in the same house and region. The periodicities of human B. malayi and feline B. malayi were similar as well as the results of Giemsa and acid phosphatase stained blood films of microfilaria positive cases. Likewise, the PCR-RFLP profile of Hha I repeat genes and PCR amplification of Trans-Spliced Leader Exon I (SLX) demonstrated that 15 samples the feline B. malayi were the same as those of human B. malayi. The data indicated that domestic cat plays an important role as the animal reservoir for B. malayi in the endemic areas of Thailand. © 2002 Elsevier Science Ltd.

Subject(s)

Acid phosphatase
 Analytic method
 Animal experiment
 Animal model
 Article
 Blood sampling
 Brugia malayi
 Cat
 Controlled study
 Exon
 Infection control
 Microfilaria
 Nonhuman
 Periodicity
 Polymerase chain reaction
 Priority journal
 Restriction fragment length polymorphism
 Risk assessment
 Thailand
 Zoonosis
 Animal
 Animal disease
 Brugia malayi
 Cat disease
 Classification
 Disease carrier
 Endemic disease
 Filariasis
 Genetics
 Human
 Isolation and purification
 Methodology
 Microfilaria
 Parasitemia
 Parasitology
 Thailand
 Zoonosis
 Animalia
 Brugia
 Brugia malayi
 Felidae
 Felis catus
 Animals
 Brugia malayi
 Cat Diseases
 Cats
 Disease Reservoirs
 Endemic Diseases
 Filariasis
 Humans
 Microfilaria
 Parasitemia
 Polymerase Chain Reaction
 Thailand
 Zoonoses

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URI

https://hdl.handle.net/20.500.14740/6787

Collections

Scopus: Year 1983-2021

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