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Identification of Vibrio spp. in vibriosis Penaeus vannamei using developed monoclonal antibodies

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dc.contributor.authorLongyant S.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorSuksawad P.
dc.contributor.authorSrisuk C.
dc.contributor.authorSithigorngul W.
dc.contributor.authorPiyatiratitivorakul S.
dc.contributor.authorSithigorngul P.
dc.date.accessioned2021-04-05T04:32:00Z
dc.date.available2021-04-05T04:32:00Z
dc.date.issued2008
dc.date.issuedBE2551
dc.description.abstractImmunohistochemical study using monoclonal antibodies specific to various shrimp viruses and Vibrio spp. was performed in shrimp samples died from unknown cause with symptoms of black stripes on lateral sides of cephalothorax or smoky body coloration. The positive results in muscular tissue were obtained with MAb VAL57 (specific to Vibrio spp.) and in hepatopancreas tissues with MAbs VVB158 (specific to V. vulnificus) and VPC701 (specific to V. parahaemolyticus). Twelve isolates of Vibrio spp. isolated from shrimp tissues were identified with various MAbs by dot blotting, biochemical tests and 16S rRNA gene. The results revealed three groups of V. vulnificus and one group of V. shilonii. All four groups of isolated Vibrio spp. were immunologically and biochemically different. None of the V. parahaemolyticus-like bacterium was isolated. The results demonstrated that the mortality in shrimp is accompanied by the presence of Vibrio spp. © 2007 Elsevier Inc. All rights reserved.
dc.format.mimetypeapplication/pdf
dc.identifier.citationJournal of Invertebrate Pathology. Vol 98, No.1 (2008), p.63-68
dc.identifier.doi10.1016/j.jip.2007.10.013
dc.identifier.issn222011
dc.identifier.other2-s2.0-42649101325
dc.identifier.urihttps://hdl.handle.net/20.500.14740/4042
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherBacterial antigen
dc.subject.otherMonoclonal antibody
dc.subject.otherAnimal
dc.subject.otherAquaculture
dc.subject.otherArticle
dc.subject.otherBacterial gene
dc.subject.otherClassification
dc.subject.otherGenetics
dc.subject.otherImmunology
dc.subject.otherMicrobiology
dc.subject.otherPathogenicity
dc.subject.otherPenaeidae
dc.subject.otherRNA gene
dc.subject.otherVibrio
dc.subject.otherAnimals
dc.subject.otherAntibodies, Monoclonal
dc.subject.otherAntigens, Bacterial
dc.subject.otherAquaculture
dc.subject.otherGenes, Bacterial
dc.subject.otherGenes, rRNA
dc.subject.otherPenaeidae
dc.subject.otherVibrio
dc.subject.otherBacteria (microorganisms)
dc.subject.otherDecapoda (Crustacea)
dc.subject.otherLitopenaeus vannamei
dc.subject.otherVibrio
dc.subject.otherVibrio parahaemolyticus
dc.subject.otherVibrio shilonii
dc.subject.otherVibrio vulnificus
dc.titleIdentification of Vibrio spp. in vibriosis Penaeus vannamei using developed monoclonal antibodies
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-42649101325&doi=10.1016%2fj.jip.2007.10.013&partnerID=40&md5=819bfcb563f3a482a1e0c0f64f40c4de

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