Publication: Cytotoxic and Genotoxic Potential of Trigonostemon reidioides Extract on Human Caco-2 Cells
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Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85074993734
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S61-S68
Suggested Citation
Nusuetrong P., Boonmalert M., Koobkokkruad T., Chantong B., Nalinratana N., Meksuriyen D. Cytotoxic and Genotoxic Potential of Trigonostemon reidioides Extract on Human Caco-2 Cells. Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S61-S68. Retrieved from: https://hdl.handle.net/20.500.14740/4506
Abstract
Background: Trigonostemon reidioides roots have been used as a Thai traditional medicine for the treatment of drug addiction; however, the cytotoxic and genotoxic potential of the herbal extract on human cells have not been clarified. Objective: To evaluate the cytotoxicity and genotoxicity of the ethanolic extract of T. reidioides roots (TR) on human intestinal epithelial Caco-2 cells. Material and Method: Cell viability of TR (0.005 to 0.5 mg/mL)-treated Caco-2 cells was measured by MTT assay after 24 and 48 h exposure. DNA fragmentation was evaluated by Hoechst 33342 staining and comet assay was conducted in the cells treated with TR at the concentrations equal to and higher than the IC50. The protein expressions of ERK1/2 as well as pERK1/ 2 were investigated by immunoblotting. Densitometric analysis of thin layer chromatography (TLC) fingerprint of TR was performed to identify scopoletin content. Results: Exposure of the human Caco-2 cells to TR resulted in decreased cell viability with an IC50 value of approximately 0.2 mg/mL. Treatment of the cells with TR (0.01 mg/mL) for 24 h and 48 h significantly increased (p<0.05) cell proliferation. TR at the concentration of IC50 induced DNA fragmentation, and TR (0.2 to 0.5 mg/mL) significantly increased the percentage of DNA in the tail in a concentration-dependent manner as revealed by comet assay. Immunoblotting revealed that the phosphorylation of ERK1/2 was significantly increased (p<0.05) in the cells treated with TR (0.5 mg/mL) when compared with the control. Scopoletin content in TLC was approximately 271 ng per mg of extract. Conclusion: The present study contributes current evidence that caution must be practiced concerning the dose and duration of T. reidioides root medication. Moreover, further study in animal models concerning its safety application is required to confirm the finding in the cellular level of the present study. © 2017 Medical Association of Thailand. All rights reserved.
Subject(s)
Cytotoxic agent
Hoe 33342
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Phytochemical
Plant extract
Trigonostemon reidioide extract
Unclassified drug
Article
Caco-2 cell line
Cell proliferation
Cell viability
Comet assay
Cytotoxicity
Densitometry
DNA fragmentation
Genotoxicity
Human
Human cell
IC50
Immunoblotting
MTT assay
Protein expression
Protein phosphorylation
Thin layer chromatography
Western blotting
Hoe 33342
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Phytochemical
Plant extract
Trigonostemon reidioide extract
Unclassified drug
Article
Caco-2 cell line
Cell proliferation
Cell viability
Comet assay
Cytotoxicity
Densitometry
DNA fragmentation
Genotoxicity
Human
Human cell
IC50
Immunoblotting
MTT assay
Protein expression
Protein phosphorylation
Thin layer chromatography
Western blotting