Publication: Focal potassium microinjection in rat hippocampal slices inducing interictal activities
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Issued Date
2011
Resource Type
File Type
application/pdf
Other identifier(s)
2-s2.0-84860442705
Rights Holder(s)
Scopus
Bibliographic Citation
BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), p.269-272
Suggested Citation
Parittotokkaporn T., Sirirattanapan J., Yu P., Hsiao M.-C., Song D., Berger T.W. Focal potassium microinjection in rat hippocampal slices inducing interictal activities. BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), p.269-272. doi:10.1109/BMEiCon.2012.6172068 Retrieved from: https://hdl.handle.net/20.500.14740/7188
Abstract
Focal potassium microinjection induces the incidence of epileptiform activities of rat hippocampal slices. We investigated the seizure responses of this activity in vitro using extracellular records from acute rat hippocampal slice. The electrophysiological recordings were performed on CA3 regions during microinjection of high potassium chloride. High potassium (8.5 mM) induces interictal field potentials generated spontaneously in the injected region of CA3. High-frequency bursting analogous to ictal seizure activity was locally obtained in the slice regarding to changes in cellular excitability synaptic signaling of potassium channel which may contribute to this phenomenon equivalent to the potassium bathed method. This study concludes that the focal potassium microinjection significantly initiates seizure at CA3 region and is a suitable model for investigating focal epileptogenesis in hippocampal slice. © 2011 IEEE.
Subject(s)
Electrophysiological recordings
Epilepsy
Epileptiform activity
Epileptogenesis
Extracellular
Field potential
High frequency HF
Hippocampal slice
In-vitro
Interictal burst
Micro-injection
Potassium channels
Potassium chloride
Seizure activity
Biomedical engineering
Chlorine compounds
Comminution
Electrophysiology
Rats
Potassium
Epilepsy
Epileptiform activity
Epileptogenesis
Extracellular
Field potential
High frequency HF
Hippocampal slice
In-vitro
Interictal burst
Micro-injection
Potassium channels
Potassium chloride
Seizure activity
Biomedical engineering
Chlorine compounds
Comminution
Electrophysiology
Rats
Potassium