Publication: In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from thai infants
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Issued Date
2018
Resource Type
File Type
application/pdf
ISSN
1253395
Other identifier(s)
2-s2.0-85044116832
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Songklanakarin Journal of Science and Technology. Vol 40, No.1 (2018), p.69-74
Suggested Citation
Nantavisai K., Puttikamonkul S., Chotelersak K., Taweechotipatr M. In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from thai infants. Songklanakarin Journal of Science and Technology. Vol 40, No.1 (2018), p.69-74. doi:10.14456/sjst-psu.2018.14 Retrieved from: https://hdl.handle.net/20.500.14740/3851
Abstract
Adhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, the adhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. casei MSMC39-3, L. salivarius MSMC105-3 and L. plantarum MSMC171-1, was studied using Caco-2 cell line as an in vitro model for intestinal epithelium. Among four different Lactobacillus strains, L. salivarius MSMC105-3 was the most adhesive strain showing about 3.5 percent of adhesion index. Thus, this strain was selected to examine for its ability to inhibit the adhesion of pathogenic Salmonella Typhi DMST5784 and Shigella dysenteriae DMST15111 to Caco-2 cells. The results showed that L. salivarius MSMC105-3 whole cell and its cell-free culture supernatant could inhibit the adhesion of pathogens. The results from this study indicated that both L. salivarius MSMC105-3 itself and its substances secreted into culture supernatant had the ability to reduce the adhesion of enteropathogens to Caco-2 cells. © 2018, Prince of Songkla University. All rights reserved.