Publication:
Interphase-FISH screening for eight common rearrangements in pediatric B-cell precursor acute lymphoblastic leukemia

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

17515521

DOI

10.1111/ijlh.12031

Other identifier(s)

2-s2.0-84880621984

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

International Journal of Laboratory Hematology. Vol 35, No.4 (2013), p.406-415

Suggested Citation

Hutspardol S., Pakakasama S., Kanta K., Nuntakarn L., Anurathapan U., Sirachainan N., Songdej D., Sawangpanich R., Tiyasirichokchai R., Rerkamnuaychoke B., Hongeng S. Interphase-FISH screening for eight common rearrangements in pediatric B-cell precursor acute lymphoblastic leukemia. International Journal of Laboratory Hematology. Vol 35, No.4 (2013), p.406-415. doi:10.1111/ijlh.12031 Retrieved from: https://hdl.handle.net/20.500.14740/6606

Title

Interphase-FISH screening for eight common rearrangements in pediatric B-cell precursor acute lymphoblastic leukemia

Author(s)

Hutspardol S.
 Pakakasama S.
 Kanta K.
 Nuntakarn L.
 Anurathapan U.
 Sirachainan N.
 Songdej D.
 Sawangpanich R.
 Tiyasirichokchai R.
 Rerkamnuaychoke B.
 Hongeng S.

Abstract

Summary: Introduction: This is the first pilot study to screen multiple common genetic aberrations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Methods: Thirty-two children with BCP-ALL were investigated for chromosomal rearrangements using interphase fluorescence in situ hybridization (FISH). Eight common translocations and rearrangements, including ETV6-RUNX1, TCF3-PBX1, BCR-ABL1, ETV6, TCF3, MLL, IGH@, and PAX5, were tested for using dual-color DNA probes. Results: ETV6-RUNX1 was the most frequent translocation detected in 11 children (34.4%). Two patients with BCR-ABL1 (6.3%) and one with TCF3-PBX1 (3.1%) translocations were also observed. Using break-apart probes, 11 children (34.4%) had a positive FISH result for ETV6, two patients for IGH@ (6.3%), one patient for MLL (3.1%), and one patient for PAX5 rearrangements (3.1%). All patients with the ETV6-RUNX1 fusion were also identified by split signals for ETV6. Other abnormalities, including extra copies and deletion of genes, were observed within the range of 3.1-34.4%. Cytogenetics analysis showed a single case each of BCR-ABL1 fusion, MLL, and IGH@ rearrangements (3.1% each). ETV6-RUNX1 fusion and ETV6 split-apart rearrangements were not visible by cytogenetics. Likewise, one each of cases with TCF3-PBX1 fusion and with PAX5 split signal seen by FISH was not visible by cytogenetics. Conclusion: By using 8 FISH probes in conjunction cytogenetics for the detection of common aberrations, interphase FISH enhanced the detection of chromosomal rearrangements in children with BCP-ALL. © 2012 John Wiley & Sons Ltd.

Subject(s)

Abelson kinase
 Breakpoint cluster region protein
 Immunoglobulin heavy chain
 Mixed lineage leukemia protein
 Transcription factor 7 like 1
 Transcription factor ETV6
 Transcription factor PAX5
 Transcription factor PBX1
 Transcription factor RUNX1
 Acute lymphoblastic leukemia
 Article
 Child
 Chromosome rearrangement
 Clinical article
 Cytogenetics
 DNA probe
 Female
 Fluorescence in situ hybridization
 Fusion gene
 Gene deletion
 Gene translocation
 Human
 Infant
 Interphase
 Male
 Pre B lymphocyte
 Preschool child
 Priority journal
 School child
 Screening
 ALL
 B-cells
 FISH
 Acute Disease
 Adolescent
 B-Lymphocytes
 Child
 Child, Preschool
 Female
 Genetic Testing
 Humans
 In Situ Hybridization, Fluorescence
 Infant
 Interphase
 Karyotyping
 Male
 Oncogene Proteins, Fusion
 Pilot Projects
 Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
 Translocation, Genetic

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URI

https://hdl.handle.net/20.500.14740/6606

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Scopus: Year 1983-2021

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